RAD23A polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot showing RAD23A polyclonal antibody (Cat # PAB10057) detects endogenous human RAD23A. Reactivity is shown against HeLa nuclear extract (Lane 1) and HeLa (Lane 2), A-431 (Lane 3), Jurkat (Lane 4) and 293 whole cell lysates (Lane 5). Indicates a band of ~60 kDa corresponding to RAD23A. The blot was incubated with a 1 : 500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG and chemiluminescence reagent with a 30-min exposure time.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of RAD23A.
Immunogen
A synthetic peptide corresponding to amino acids 122-135 of human RAD23A.
Host
Goat
Reactivity
Human, Rabbit
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000-1:16000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot showing RAD23A polyclonal antibody (Cat # PAB10057) detects endogenous human RAD23A. Reactivity is shown against HeLa nuclear extract (Lane 1) and HeLa (Lane 2), A-431 (Lane 3), Jurkat (Lane 4) and 293 whole cell lysates (Lane 5). Indicates a band of ~60 kDa corresponding to RAD23A. The blot was incubated with a 1 : 500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG and chemiluminescence reagent with a 30-min exposure time.Enzyme-linked Immunoabsorbent Assay
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Gene Info — RAD23A
Entrez GeneID
5886Protein Accession#
P54725;NP_005044Gene Name
RAD23A
Gene Alias
HHR23A, MGC111083
Gene Description
RAD23 homolog A (S. cerevisiae)
Omim ID
600061Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq
Other Designations
RAD23, yeast homolog, A|UV excision repair protein RAD23 homolog A
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Interactome
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Pathway
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Disease
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Publication Reference
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Binding of polyubiquitin chains to ubiquitin-associated (UBA) domains of HHR23A.
Raasi S, Orlov I, Fleming KG, Pickart CM.
Journal of Molecular Biology 2004 Aug; 341(5):1367.
Application：WB, Chemical.
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Involvement of the DNA repair protein hHR23 in p53 degradation.
Glockzin S, Ogi FX, Hengstermann A, Scheffner M, Blattner C.
Molecular and Cellular Biology 2003 Dec; 23(24):8960.
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DNA-repair protein hHR23a alters its protein structure upon binding proteasomal subunit S5a.
Walters KJ, Lech PJ, Goh AM, Wang Q, Howley PM.
PNAS 2003 Oct; 100(22):12694.
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Binding of polyubiquitin chains to ubiquitin-associated (UBA) domains of HHR23A.
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