Western blot showing RAD23A polyclonal antibody (Cat # PAB10057) detects endogenous human RAD23A. Reactivity is shown against HeLa nuclear extract (Lane 1) and HeLa (Lane 2), A-431 (Lane 3), Jurkat (Lane 4) and 293 whole cell lysates (Lane 5). Indicates a band of ~60 kDa corresponding to RAD23A. The blot was incubated with a 1 : 500 dilution of the antibody at room temperature followed by detection using HRP conjugated Rb-a-Goat IgG and chemiluminescence reagent with a 30-min exposure time.
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. [provided by RefSeq
RAD23, yeast homolog, A,UV excision repair protein RAD23 homolog A