GSK3B (phospho S9) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) at a 1:1,000 dilution.
All lanes contain human 293T whole cell lysate showing a band at 47 KDa.
Cells were serum starved for 24 h prior to extraction.
Key : Lane 1, Control; Lane 2, treated with IGF-1 (100 ng/mL) for 20'; lane 3, pre-treated with 10 uM selective PI3K inhibitor and treated with IGF-1 (100 ng/mL) for 20'.
Molecular weight markers confirm a MW of ~ 49 KDa.
Use a 1 : 2,000 dilution of HRP Goat-a-Rabbit IgG for detection.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) was used at a 1 : 200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3B.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S9 of human GSK3B.
Host
Rabbit
Reactivity
Chicken, Dog, Frog, Human, Rat
Specificity
This phospho specific polyclonal antibody reacts with phosphorylated pS9 of human GSK3B. Reactivity with non-phosphorylated human GSK3B is minimal.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:3000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) at a 1:1,000 dilution.
All lanes contain human 293T whole cell lysate showing a band at 47 KDa.
Cells were serum starved for 24 h prior to extraction.
Key : Lane 1, Control; Lane 2, treated with IGF-1 (100 ng/mL) for 20'; lane 3, pre-treated with 10 uM selective PI3K inhibitor and treated with IGF-1 (100 ng/mL) for 20'.
Molecular weight markers confirm a MW of ~ 49 KDa.
Use a 1 : 2,000 dilution of HRP Goat-a-Rabbit IgG for detection.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of GSK3B (phospho S9) polyclonal antibody (Cat # PAB10055) was used at a 1 : 200 dilution to detect GSK3B by immunohistochemistry in human ovarian cancer tumor tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — GSK3B
Entrez GeneID
2932Protein Accession#
NM_002093(Human);P49841(Human)Gene Name
GSK3B
Gene Alias
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Gene Description
glycogen synthase kinase 3 beta
Omim ID
605004Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene
Other Designations
GSK3beta isoform|glycogen synthase kinase-3 beta
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Interactome
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Pathway
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Disease
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Publication Reference
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Insulin ameliorates dim blue light at night-induced apoptosis in hippocampal neurons via the IR/IRS1/AKT/GSK3β/β-catenin signaling pathway.
Qi Liu, Zixu Wang, Jing Cao, Yulan Dong, Yaoxing Chen.
Ecotoxicology and Environmental Safety 2022 Dec; 250:114488.
Application：WB-Ce, WB-Ti, Mouse, Mouse hippocampus, HT22 cells.
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A Green and Blue Monochromatic Light Combination Therapy Reduces Oxidative Stress and Enhances B-Lymphocyte Proliferation through Promoting Melatonin Secretion.
Yijia Zhang, Zixu Wang, Jing Cao, Yulan Dong, Yaoxing Chen.
Oxidative Medicine and Cellular Longevity 2021 Mar; 2021:5595376.
Application：WB-Ti, Chicken, Whole bursa of Fabricius.
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The structure of phosphorylated GSK-3beta complexed with a peptide, FRATtide, that inhibits beta-catenin phosphorylation.
Bax B, Carter PS, Lewis C, Guy AR, Bridges A, Tanner R, Pettman G, Mannix C, Culbert AA, Brown MJ, Smith DG, Reith AD.
Structure 2001 Dec; 9(12):1143.
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Crystal structure of glycogen synthase kinase 3 beta: structural basis for phosphate-primed substrate specificity and autoinhibition.
Dajani R, Fraser E, Roe SM, Young N, Good V, Dale TC, Pearl LH.
Cell 2001 Jun; 105(6):721.
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Cloning and characterization of a novel human ninein protein that interacts with the glycogen synthase kinase 3beta.
Hong YR, Chen CH, Chang JH, Wang S, Sy WD, Chou CK, Howng SL.
Biochimica et Biophysica Acta 2000 Jul; 1492(2-3):513.
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Insulin ameliorates dim blue light at night-induced apoptosis in hippocampal neurons via the IR/IRS1/AKT/GSK3β/β-catenin signaling pathway.
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