Product Browser

Last updated: 2017/6/18
  • Related Product Showcase

Product Compare

Product Compare Cancel Click this icon to add products to compare list. Select up to 10 products.

Quick Order (Tutorial)

Input Catalog #,
place order here!
Catalog # :
  • Where to buy
  • Choose your location

GSK3A (phospho S21) polyclonal antibody

  • Catalog # : PAB10054
  • Visit Frequency :
  • Countries :
  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of GSK3A.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S21 of human GSK3A.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Human, Rat
  • Specificity:
  • This phospho specific polyclonal antibody reacts with phosphorylated pS21 of human GSK3A. Reactivity with non-phosphorylated human GSK3A is minimal.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:2000-1:10000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using GSK3A (phospho S21) polyclonal antibody (Cat # PAB10054) shows detection of a 52 KDa band corresponding to human GSK3A in various human derived 293T cell extracts.
    Cells were serum starved for 24 h.
    Lane 1 : control, Lane 2: treated with IGF-1 (100 ng/mL) for 20 min, Lane 3 : pre-treated with 10 uM PI3K inhibitor and treated with IGF-1 (100 ng/mL) for 20 min.
    Lane 1 shows some baseline GSK3A reactivity that is intensified upon stimulation (lane2) and diminished when aninhibitor is added (Lane 3).
    Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocel followed by reaction with a 1:1,000 dilution of anti-GSK3A antibody.
    Signal was detected using standard techniques.
    Personal communication, Angela Carter, Experimental Therapeutics, Ontario Cancer Inst, Toronto, Canada.
  • Immunofluorescence
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 2931
  • Protein Accession#:
  • P49840;NP_063937
  • Gene Name:
  • GSK3A
  • Gene Alias:
  • DKFZp686D0638
  • Gene Description:
  • glycogen synthase kinase 3 alpha
  • Gene Summary:
  • Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM
  • Other Designations:
  • -
  • RSS
  • YouTube
  • Linkedin
  • Facebook