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Last updated: 2016/12/4
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GGA3 polyclonal antibody

  • Catalog # : PAB10050
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of GGA3.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 400-415 of human GGA3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Macaque, Orangutan
  • Specificity:
  • The immunizing sequence is present on both the long (isoform 1) and short (isoform 2) transcript variants for this protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:500-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot using GGA3 polyclonal antibody (Cat # PAB10050) shows detection of aband at ~110 KDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cellsover- expressing the recombinant protein (Lane 2, arrowhead).
    Pre-incubation of antibody with immunizing peptide blocks specific staining (lane3).
    MW markers are shown in lane 1 (700 nm channel, red).
    Approximately 35 ug of lysate was separated on a 16% Tricine gel by SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1:600.
    Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q9NZ52;NP_619525
  • Gene Name:
  • GGA3
  • Gene Alias:
  • KIAA0154
  • Gene Description:
  • golgi associated, gamma adaptin ear containing, ARF binding protein 3
  • Gene Summary:
  • This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq
  • Other Designations:
  • ADP-ribosylation factor binding protein 3,Golgi-localized, gamma ear-containing, ARF-binding protein 3
  • Gene Pathway
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