Western blot using GGA3 polyclonal antibody (Cat # PAB10050) shows detection of aband at ~110 KDa corresponding to GFP-GGA3 fusion protein present in a lysate of HEK293 cellsover- expressing the recombinant protein (Lane 2, arrowhead). Pre-incubation of antibody with immunizing peptide blocks specific staining (lane3). MW markers are shown in lane 1 (700 nm channel, red). Approximately 35 ug of lysate was separated on a 16% Tricine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:600. Reaction occurred overnight at 4°C followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR. IRDye is a trademark of LI-COR, Inc.
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Alternative splicing of this gene results in two transcript variants. [provided by RefSeq
ADP-ribosylation factor binding protein 3,Golgi-localized, gamma ear-containing, ARF-binding protein 3