FANCG polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using FANCG polyclonal antibody (Cat # PAB10048) shows detection of a band at ~69 KDa (arrowhead) corresponding to FANCG present in a HeLa whole cell lysate.
Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers (indicated at left).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of FANCG polyclonal antibody (Cat # PAB10048) shows strong nuclear and cytoplasmic staining of cells of macrophages in human lung tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FANCG.
Immunogen
A synthetic peptide corresponding to amino acids 1-12 of human FANCG.
Host
Rabbit
Reactivity
Chimpanzee, Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using FANCG polyclonal antibody (Cat # PAB10048) shows detection of a band at ~69 KDa (arrowhead) corresponding to FANCG present in a HeLa whole cell lysate.
Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500.
The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers (indicated at left).
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of FANCG polyclonal antibody (Cat # PAB10048) shows strong nuclear and cytoplasmic staining of cells of macrophages in human lung tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Uppsala, Sweden.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FANCG
Entrez GeneID
2189Protein Accession#
NP_004620;O15287Gene Name
FANCG
Gene Alias
FAG, XRCC9
Gene Description
Fanconi anemia, complementation group G
Omim ID
602956Gene Ontology
HyperlinkGene Summary
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group G. [provided by RefSeq
Other Designations
DNA repair protein XRCC9|OTTHUMP00000021319|X-ray repair complementing defective repair in Chinese hamster cells 9|X-ray repair, complementing defective, in Chinese hamster, 9
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Interactome
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Disease
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Publication Reference
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Telomere dynamics in Fancg-deficient mouse and human cells.
Franco S, van de Vrugt HJ, Fernández P, Aracil M, Arwert F, Blasco MA.
Blood 2004 Dec; 104(13):3927.
Application：IP, WB, Human, Mouse, HEK 293 cells, MEFs.
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The Fanconi anemia core complex associates with chromatin during S phase.
Mi J, Kupfer GM.
Blood 2005 Jan; 105(2):759.
Application：WB, Human, HeLa cells.
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Oxidative stress/damage induces multimerization and interaction of Fanconi anemia proteins.
Park SJ, Ciccone SL, Beck BD, Hwang B, Freie B, Clapp DW, Lee SH.
The Journal of Biological Chemistry 2004 May; 279(29):30053.
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Telomere dynamics in Fancg-deficient mouse and human cells.
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