Western blot analysis is shown using SETDB1 polyclonal antibody (Cat # PAB10044) to detect human SETBD1 present in a 293 whole cell lysate. Approximately 30 ug of lysate was loaded per lane for SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a singleband of ~170 KDa is detected. Peptide competition (not shown) blocks staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted1:5,000 for 45 min. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.
This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described