Products

Western Blot (Cell lysate)

Western blot analysis is shown using SETDB1 polyclonal antibody (Cat # PAB10044) to detect human SETBD1 present in a 293 whole cell lysate.
Approximately 30 ug of lysate was loaded per lane for SDS-PAGE.
Comparison to a molecular weight marker (not shown) indicates a singleband of ~170 KDa is detected.
Peptide competition (not shown) blocks staining of this band.
The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted1:5,000 for 45 min.
IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.

Immunohistochemistry

Enzyme-linked Immunoabsorbent Assay

Entrez GeneID

9869

Protein Accession#

NP_036564;Q15047

Gene Name

SETDB1

Gene Alias

ESET, KG1T, KIAA0067, KMT1E

Gene Description

SET domain, bifurcated 1

Omim ID

604396

Gene Ontology

Hyperlink

Gene Summary

This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described

Other Designations

ERG-associated protein with a SET domain, ESET