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Last updated: 2016/12/4
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SETDB1 polyclonal antibody

  • Catalog # : PAB10044
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of SETDB1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 1058-1075 of human SETDB1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:1000-1:2500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis is shown using SETDB1 polyclonal antibody (Cat # PAB10044) to detect human SETBD1 present in a 293 whole cell lysate.
    Approximately 30 ug of lysate was loaded per lane for SDS-PAGE.
    Comparison to a molecular weight marker (not shown) indicates a singleband of ~170 KDa is detected.
    Peptide competition (not shown) blocks staining of this band.
    The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2h followed by detection using IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted1:5,000 for 45 min.
    IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunohistochemistry
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 9869
  • Protein Accession#:
  • NP_036564;Q15047
  • Gene Name:
  • SETDB1
  • Gene Alias:
  • ESET,KG1T,KIAA0067,KMT1E
  • Gene Description:
  • SET domain, bifurcated 1
  • Gene Summary:
  • This gene encodes a histone methyltransferase. The encoded enzyme catalyzes the reaction of S-adenosyl-L-methionine and histone L-lysine to produce S-adenosyl-L-homocysteine and histone N(6)-methyl-L-lysine. The encoded protein likely functions in transcriptional repression. Alternatively spliced transcript variants have been described
  • Other Designations:
  • ERG-associated protein with a SET domain, ESET
  • Gene Pathway
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