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Last updated: 2016/12/4
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CCNB1 (phospho S126) polyclonal antibody

  • Catalog # : PAB10042
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of CCNB1.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding S126 of human CCNB1.
  • Host:
  • Rabbit
  • Reactivity:
  • Chimpanzee, Dog, Human, Rat
  • Specificity:
  • This antibody is specific to phosphorylated human Cyclin B1 protein at the pS126 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:50000)
    Western Blot (1:100-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using CCNB1 (phospho S126) polyclonal antibody (Cat # PAB10042) shows detection of a band ~48 kDa corresponding to phosphorylated human CCNB1 (arrowheads) in various whole cell lysates.
    Lysates tested were lane 1 - Hela (cervical carcinoma), lane 2 - H23 (lung carcinoma), lane 3 - Hep3b (Hepatocarcinoma), lane 4 - T98G (Glioblastoma) and lane 5 - Daudi (B cell lymphoblast).
    Each lane contains approximately 50 ug of lysates, separated by 12% SDS-PAGE using a 5% stack run at 100 volts until the dye front cleared the bottom of the gel.
    Transfer occurred overnight at 4°C at 15 mAmps.
    The membrane was blocked with 5% non-fat dry milk in TTBS for 1 h at room temperature followed by addition of a 1:100 dilution of the antibody allowed to react for 2h at room temperature.
    After washes with TTBS a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG [H&L] MX was added for 1 h at room temperature.
    After additional washes the membrane was incubated with ECL mix 1:
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 891
  • Protein Accession#:
  • P14635;NP_114172
  • Gene Name:
  • CCNB1
  • Gene Alias:
  • CCNB
  • Gene Description:
  • cyclin B1
  • Gene Summary:
  • The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq
  • Other Designations:
  • G2/mitotic-specific cyclin B1
  • Interactome
  • Interactome
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