CUL7 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CUL7 polyclonal antibody (Cat # PAB10040) was diluted 1 : 500 to detect CUL7 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CUL7.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 1679-1698 of human CUL7.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CUL7 polyclonal antibody (Cat # PAB10040) was diluted 1 : 500 to detect CUL7 in human brain cortex tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CUL7
Entrez GeneID
9820Protein Accession#
Q14999;NP_055595Gene Name
CUL7
Gene Alias
KIAA0076, dJ20C7.5
Gene Description
cullin 7
Gene Ontology
HyperlinkOther Designations
OTTHUMP00000016418
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Interactome
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Pathway
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Publication Reference
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Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis.
Arai T, Kasper JS, Skaar JR, Ali SH, Takahashi C, DeCaprio JA.
PNAS 2003 Aug; 100(17):9855.
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CUL7: A DOC domain-containing cullin selectively binds Skp1.Fbx29 to form an SCF-like complex.
Dias DC, Dolios G, Wang R, Pan ZQ.
PNAS 2002 Dec; 99(26):16601.
Application：WB, Human, HEK 293 cells.
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Ubiquitin and its kin: how close are the family ties?
Jentsch S, Pyrowolakis G.
Trends in Cell Biology 2000 Aug; 10(8):335.
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Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis.
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