CUL5 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CUL5 polyclonal antibody (Cat # PAB10039) was diluted 1 : 500 to detect CUL5 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CUL5.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 4-18 of human CUL5.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining with CUL5 polyclonal antibody (Cat # PAB10039) was diluted 1 : 500 to detect CUL5 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — CUL5
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex.
Yu X, Yu Y, Liu B, Luo K, Kong W, Mao P, Yu XF.
Science 2003 Nov; 302(5647):1056.
Application：EWB-Tr, Human, H9, 293T cells.
-
Degradation of p53 by adenovirus E4orf6 and E1B55K proteins occurs via a novel mechanism involving a Cullin-containing complex.
Querido E, Blanchette P, Yan Q, Kamura T, Morrison M, Boivin D, Kaelin WG, Conaway RC, Conaway JW, Branton PE.
Genes & Development 2001 Dec; 15(23):3104.
Application：WB-Tr, Human, H1299 cells.
-
ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity.
Ohta T, Michel JJ, Schottelius AJ, Xiong Y.
Molecular Cell 1999 Apr; 3(4):535.
-
Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A.
Michel JJ, Xiong Y.
Cell Growth & Differentiation: the Molecular Biology Journal of the American Association for Cancer 1998 Jun; 9(6):435.
-
Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins.
Mathias N, Johnson SL, Winey M, Adams AE, Goetsch L, Pringle JR, Byers B, Goebl MG.
Molecular and Cellular Biology 1996 Dec; 16(12):6634.
Application：WB, Yeast, Yeast cells.
-
Induction of APOBEC3G ubiquitination and degradation by an HIV-1 Vif-Cul5-SCF complex.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com