Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK2.
Synthetic phosphopeptide corresponding to residues surrounding T68 of human CHEK2.
Reactivity occurs against human CHK2 pT68 protein and This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human CHK2 is minimal by ELISA. This antibody does not cross-react with Chk2 phosphorylated at other sites.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:10000-1:50000) Western Blot (1:200-1:2000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using CHEK2 (phospho T68) polyclonal antibody (Cat # PAB10034) shows detection of apredominant band at ~60 kDa corresponding to phosphorylated CHEK2 (arrowhead) in MCF-7 whole cell lysates after treatment with doxorubicin. CHEK2 phosphorylation was induced using increasing concentrations of the DNA damaging agent doxorubicin as indicated for 24 h prior to lysate production. Personal communication, Xiao HeYang, University of Oklahoma Health Sciences Center.
In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
OTTHUMP00000028871,checkpoint-like protein CHK2,protein kinase CHK2,serine/threonine-protein kinase CHK2