ANAPC1 (phospho S355) polyclonal antibody

Catalog # PAB10017

Size

Price

Stock

Quantity

Size:100 ug
Price: USD $ 638.00
Stock:
order now, ship in 5 days
abnova-minus
abnova-plus

* The price is valid only in USA. Please select country.

Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot (Cell lysate)
Application

Western Blot (Cell lysate)

Western blot using ANAPC1 (phospho S355) polyclonal antibody (Cat # PAB10017) shows detection of a band ~215 KDa corresponding to phosphorylated human ANAPC1 (arrowhead).
Lane 1 shows lysate from asynchronous cells.
Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary.
Lane 3 shows lysate from cells treated with nocodazole to synchronizecells at the M phase.
Phosphorylated ANAPC1 is mostly present only in cell preparations arrested at cell division.
Each lane contains approximately 30 ug of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose.
After blocking with 5% non-fat drymilk in TTBS, the membrane was probed with the primary antibody diluted to 1 : 500 for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
ECL reagent was used for detection.
Data contributed by Bing Li, UT South western.

  • Specifications

    Product Description

    Rabbit polyclonal antibody raised against synthetic phosphopeptide of ANAPC1.

    Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding S355 of human ANAPC1.

    Host

    Rabbit

    Reactivity

    Bovine, Chicken, Chimpanzee, Dog, Human, Mouse, Rat

    Specificity

    This antibody is specific to phosphorylated human APC1 protein at the pS355 residue. Minimal reactivity is expected with the non-phosphorylated form of the protein.

    Form

    Liquid

    Quality Control Testing

    Antibody Reactive Against Synthetic Peptide.

    Recommend Usage

    ELISA (1:10000-1:40000)
    Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)

    Storage Instruction

    Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Western Blot (Cell lysate)

    Western blot using ANAPC1 (phospho S355) polyclonal antibody (Cat # PAB10017) shows detection of a band ~215 KDa corresponding to phosphorylated human ANAPC1 (arrowhead).
    Lane 1 shows lysate from asynchronous cells.
    Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary.
    Lane 3 shows lysate from cells treated with nocodazole to synchronizecells at the M phase.
    Phosphorylated ANAPC1 is mostly present only in cell preparations arrested at cell division.
    Each lane contains approximately 30 ug of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose.
    After blocking with 5% non-fat drymilk in TTBS, the membrane was probed with the primary antibody diluted to 1 : 500 for 1 h at room temperature followed by washes and reaction with a 1 : 5,000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
    ECL reagent was used for detection.
    Data contributed by Bing Li, UT South western.

    Immunoprecipitation

    Enzyme-linked Immunoabsorbent Assay

  • Gene Info — ANAPC1

    Entrez GeneID

    64682

    Protein Accession#

    Q9H1A4;NP_073153

    Gene Name

    ANAPC1

    Gene Alias

    APC1, MCPR, TSG24

    Gene Description

    anaphase promoting complex subunit 1

    Omim ID

    608473

    Gene Ontology

    Hyperlink

    Gene Summary

    ANAPC1 is 1 of at least 10 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.[supplied by OMIM

    Other Designations

    anaphase-promoting complex 1 (meiotic checkpoint regulator)

  • Interactomes
  • Pathways
  • Publication Reference
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
4 Products to Compare
Remove All