Rabbit polyclonal antibody raised against synthetic phosphopeptide of HMGN1.
Synthetic phosphopeptide corresponding to residues surrounding S20/S24 of human HMGN1.
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Orangutan, Pig, Rat
Reactivity occurs against human HMGN pS20/pS24 proteins and This antibody is specific to the phosphorylated form of the proteins. Reactivity is seen against HMGN1 and HMGN2. HMGN3 is also likely reactive although not tested. Reactivity with non-phosphorylated human HMGNs is minimal by ELISA.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:5000-1:25000 Western Blot (1:500-1:2000) Immunohistochemistry (20 ug/mL) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using HMGN1 (phospho S20/24) polyclonal antibody (Cat # PAB10016) shows detection of phosphorylated HMGN1 and HMGN2. Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues. Lanes contain : 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 Δ20E, Δ24E, non-phosphorylated, 4 - HMGN1, Δ20E, Δ24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated. Molecular weight markers (not shown) confirm the size of each recombinant protein. The primary antibody was diluted 1 : 1,000 for this experiment. The blot was processed using a 5 sec exposure time. Personal Communication, Yuri Postnikov, NIH, CCR, Bethesda, MD.
Immunohistochemistry of HMGN1 (phospho S20/24) polyclonal antibody (Cat # PAB10016) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.[supplied by OMIM
OTTHUMP00000068966,high-mobility group (nonhistone chromosomal) protein 14,high-mobility group nucleosome binding 1,nonhistone chromosomal protein HMG-14