HMGN1 (phospho S20/S24) polyclonal antibody
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More Files
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Western Blot (Recombinant protein)
Western blot using HMGN1 (phospho S20/24) polyclonal antibody (Cat # PAB10016) shows detection of phosphorylated HMGN1 and HMGN2.
Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues.
Lanes contain : 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 Δ20E, Δ24E, non-phosphorylated, 4 - HMGN1, Δ20E, Δ24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated.
Molecular weight markers (not shown) confirm the size of each recombinant protein.
The primary antibody was diluted 1 : 1,000 for this experiment.
The blot was processed using a 5 sec exposure time.
Personal Communication, Yuri Postnikov, NIH, CCR, Bethesda, MD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of HMGN1 (phospho S20/24) polyclonal antibody (Cat # PAB10016) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of HMGN1.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S20/S24 of human HMGN1.
Host
Rabbit
Reactivity
Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Orangutan, Pig, Rat
Specificity
Reactivity occurs against human HMGN pS20/pS24 proteins and This antibody is specific to the phosphorylated form of the proteins. Reactivity is seen against HMGN1 and HMGN2. HMGN3 is also likely reactive although not tested. Reactivity with non-phosphorylated human HMGNs is minimal by ELISA.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:25000
Western Blot (1:500-1:2000)
Immunohistochemistry (20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using HMGN1 (phospho S20/24) polyclonal antibody (Cat # PAB10016) shows detection of phosphorylated HMGN1 and HMGN2.
Recombinant native and mutant HMGN proteins were treated with kinase PKCalpha to specifically phosphorylate S20 and S24 residues.
Lanes contain : 1 - HMGN1, non-phosphorylated, 2 - HMGN1, phosphorylated, 3 - HMGN1 Δ20E, Δ24E, non-phosphorylated, 4 - HMGN1, Δ20E, Δ24E, phosphorylated, 5 - HMGN2, non-phosphorylated, and 6 - HMGN2, phosphorylated.
Molecular weight markers (not shown) confirm the size of each recombinant protein.
The primary antibody was diluted 1 : 1,000 for this experiment.
The blot was processed using a 5 sec exposure time.
Personal Communication, Yuri Postnikov, NIH, CCR, Bethesda, MD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of HMGN1 (phospho S20/24) polyclonal antibody (Cat # PAB10016) was used at 20 ug/mL to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
This image shows moderate nuclear and faint cytoplasmic positive staining of epidermal keratinocytes at 40X.
Tissue was formalin-fixed and paraffin embedded.
The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HMGN1
Entrez GeneID
3150Protein Accession#
AAA52677;P05114Gene Name
HMGN1
Gene Alias
FLJ27265, FLJ31471, HMG14, MGC104230, MGC117425
Gene Description
high-mobility group nucleosome binding domain 1
Omim ID
163920Gene Ontology
HyperlinkGene Summary
Chromosomal protein HMG14 and its close analog HMG17 (MIM 163910) bind to the inner side of the nucleosomal DNA, potentially altering the interaction between the DNA and the histone octamer. The 2 proteins may be involved in the process that maintains transcribable genes in a unique chromatin conformation. Their ubiquitous distribution and relative abundance, as well as the high evolutionary conservation of the DNA-binding domain of the HMG14 family of proteins, suggest that they may be involved in an important cellular function.[supplied by OMIM
Other Designations
OTTHUMP00000068966|high-mobility group (nonhistone chromosomal) protein 14|high-mobility group nucleosome binding 1|nonhistone chromosomal protein HMG-14
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Interactome
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Publication Reference
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Chromosomal protein HMG-14. Identification, characterization, and chromosome localization of a functional gene from the large human multigene family.
Landsman D, McBride OW, Soares N, Crippa MP, Srikantha T, Bustin M.
The Journal of Biological Chemistry 1989 Feb; 264(6):3421.
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Human non-histone chromosomal protein HMG-17: identification, characterization, chromosome localization and RFLPs of a functional gene from the large multigene family.
Landsman D, McBride OW, Bustin M.
Proteins 1988 Jan; 3(2):113.
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Chromosomal protein HMG-14. Identification, characterization, and chromosome localization of a functional gene from the large human multigene family.
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