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Last updated: 2016/11/27
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FBXO9 polyclonal antibody

  • Catalog # : PAB10012
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of FBXO9.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 431-447 of human FBXO9.
  • Host:
  • Rabbit
  • Reactivity:
  • Bovine, Chicken, Chimpanzee, Clawed frog, Dog, Human, Mouse, Rat
  • Specificity:
  • Reactivity is expected against all known isoforms of this protein, as the immunogen sequence is common to their carboxy-terminal ends.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:25000)
    Western Blot (1:500-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using FBXO9 polyclonal antibody (Cat # PAB10012) shows detection of a band at ~100 KDa (arrowhead) believed to correspond to FBOX9 present in a MCF-7 whole cell lysate (Lane 1).
    Specific band reactivity is greatly diminished when theantibody is pre-incubated with the immunizing peptide (data not shown).
    Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
    After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1 : 1,500.
    The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
    Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red).
    IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developed by LI-COR.
    IRDye is a trademark of LI-COR, Inc.
  • Immunofluorescence
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunofluorescence
  • ELISA
  • Gene Information
  • Protein Accession#:
  • NP_036479(isoform1);NP_258441(isoform2);NP_258442(isoform3)
  • Gene Name:
  • FBXO9
  • Gene Alias:
  • DKFZp434C0118,FBX9,KIAA0936,NY-REN-57,VCIA1,dJ341E18.2
  • Gene Description:
  • F-box protein 9
  • Gene Summary:
  • This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates at least 3 transcript variants diverging at the 5' terminus. [provided by RefSeq
  • Other Designations:
  • F-box only protein 9,F-box protein Fbx9,cross-immune reaction antigen
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