FBXO9 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FBXO9.
Immunogen
A synthetic peptide corresponding to amino acids 431-447 of human FBXO9.
Host
Rabbit
Reactivity
Bovine, Chicken, Chimpanzee, Clawed frog, Dog, Human, Mouse, Rat
Specificity
Reactivity is expected against all known isoforms of this protein, as the immunogen sequence is common to their carboxy-terminal ends.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:5000-1:25000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using FBXO9 polyclonal antibody (Cat # PAB10012) shows detection of a band at ~100 KDa (arrowhead) believed to correspond to FBOX9 present in a MCF-7 whole cell lysate (Lane 1).
Specific band reactivity is greatly diminished when theantibody is pre-incubated with the immunizing peptide (data not shown).
Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.
After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1 : 1,500.
The membrane was washed and reacted with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red).
IRDye™800 fluorescence image was captured usingthe Odyssey® Infrared Imaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — FBXO9
Entrez GeneID
26268Protein Accession#
NP_036479(isoform1);NP_258441(isoform2);NP_258442(isoform3)Gene Name
FBXO9
Gene Alias
DKFZp434C0118, FBX9, KIAA0936, NY-REN-57, VCIA1, dJ341E18.2
Gene Description
F-box protein 9
Omim ID
609091Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates at least 3 transcript variants diverging at the 5' terminus. [provided by RefSeq
Other Designations
F-box only protein 9|F-box protein Fbx9|cross-immune reaction antigen
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Interactome
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Publication Reference
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Systematic analysis and nomenclature of mammalian F-box proteins.
Jin J, Cardozo T, Lovering RC, Elledge SJ, Pagano M, Harper JW.
Genes & Development 2004 Nov; 18(21):2573.
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Antigens recognized by autologous antibody in patients with renal-cell carcinoma.
Scanlan MJ, Gordan JD, Williamson B, Stockert E, Bander NH, Jongeneel V, Gure AO, Jager D, Jager E, Knuth A, Chen YT, Old LJ.
International Journal of Cancer 1999 Nov; 83(4):456.
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A family of mammalian F-box proteins.
Winston JT, Koepp DM, Zhu C, Elledge SJ, Harper JW.
Current Biology 1999 Oct; 9(20):1180.
Application:WB-Tr, Insect cells, Skp1 protein.
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Systematic analysis and nomenclature of mammalian F-box proteins.
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