FANCF polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot using FANCF polyclonal antibody (Cat # PAB10011) shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4).
This band (indicated by arrowhead) is approximately 42.3 kDa in size.
The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2).
Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation.
No apparent difference was noted upon irradiation.
The strong band at ~60kDa appears to be non-specific.
Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of FANCF.
Immunogen
A synthetic peptide corresponding to internal region of human FANCF.
Host
Rabbit
Reactivity
Chimpanzee, Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:15000-1:60000)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using FANCF polyclonal antibody (Cat # PAB10011) shows detection of FANCF present in a lysate prepared from a Fanconi anemia complementation group F patient lymphoblast after retroviral correction using hFANCF cDNA (lanes 3 and 4).
This band (indicated by arrowhead) is approximately 42.3 kDa in size.
The band is not detected in FA-F a lymphoblast lysate that is not corrected for the deletion and does not express the FANCF protein (lanes 1 and 2).
Lanes 2 and 4 represent lysates taken from lymphoblasts after 40 J/m2 UV irradiation, whereas lanes 1 and 3 received no irradiation.
No apparent difference was noted upon irradiation.
The strong band at ~60kDa appears to be non-specific.
Personal communication, N. Howlett, University of Rhode Island, Kingston, RI.Enzyme-linked Immunoabsorbent Assay
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Gene Info — FANCF
Entrez GeneID
2188Protein Accession#
NP_073562;Q9NPI8;Q52LM0Gene Name
FANCF
Gene Alias
FAF, MGC126856
Gene Description
Fanconi anemia, complementation group F
Omim ID
603467Gene Ontology
HyperlinkGene Summary
The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear protein complex. This gene encodes the protein for complementation group F. [provided by RefSeq
Other Designations
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Interactome
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Disease
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Publication Reference
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The Fanconi anemia gene product FANCF is a flexible adaptor protein.
Leveille F, Blom E, Medhurst AL, Bier P, Laghmani el H, Johnson M, Rooimans MA, Sobeck A, Waisfisz Q, Arwert F, Patel KJ, Hoatlin ME, Joenje H, de Winter JP.
The Journal of Biological Chemistry 2004 Sep; 279(38):39421.
Application：IP, WB, Human, HSC93 cells.
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Promoter hypermethylation of FANCF: disruption of Fanconi Anemia-BRCA pathway in cervical cancer.
Narayan G, Arias-Pulido H, Nandula SV, Basso K, Sugirtharaj DD, Vargas H, Mansukhani M, Villella J, Meyer L, Schneider A, Gissmann L, Durst M, Pothuri B, Murty VV.
Cancer Research 2004 May; 64(9):2994.
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Inactivation of the Fanconi anemia/BRCA pathway in lung and oral cancers: implications for treatment and survival.
Marsit CJ, Liu M, Nelson HH, Posner M, Suzuki M, Kelsey KT.
Oncogene 2004 Jan; 23(4):1000.
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The Fanconi anemia gene product FANCF is a flexible adaptor protein.
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