EGR1 polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot using EGR1 polyclonal antibody (Cat # PAB10007) shows detectionof a predominant band at ~58 KDa correspond-ing to EGR1 present in mouse embryonic fibroblast whole cell lysate (arrowhead).
Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,500.
Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of EGR1 polyclonal antibody (Cat # PAB10007) was used at a 10 ug/mL to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining.
This image shows EGR1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of EGR1.
Immunogen
A synthetic peptide corresponding to amino acids 94-108 of human EGR1.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:4000-1:16000)
Western Blot (1:500-1:3000)
Immunohistochemistry (2-20 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot using EGR1 polyclonal antibody (Cat # PAB10007) shows detectionof a predominant band at ~58 KDa correspond-ing to EGR1 present in mouse embryonic fibroblast whole cell lysate (arrowhead).
Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,500.
Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry of EGR1 polyclonal antibody (Cat # PAB10007) was used at a 10 ug/mL to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining.
This image shows EGR1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.Enzyme-linked Immunoabsorbent Assay
-
Gene Info — EGR1
Entrez GeneID
1958Protein Accession#
P18146Gene Name
EGR1
Gene Alias
AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
Gene Description
early growth response 1
Omim ID
128990Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq
Other Designations
early growth response protein 1|nerve growth factor-induced protein A|transcription factor ETR103|zinc finger protein 225
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
A gene coding for a zinc finger protein is induced during 12-O-tetradecanoylphorbol-13-acetate-stimulated HL-60 cell differentiation.
Shimizu N, Ohta M, Fujiwara C, Sagara J, Mochizuki N, Oda T, Utiyama H.
Journal of Biochemistry 1992 Feb; 111(2):272.
-
cDNA sequence of the human cellular early growth response gene Egr-1.
Suggs SV, Katzowitz JL, Tsai-Morris C, Sukhatme VP.
Nucleic Acids Research 1990 Jul; 18(14):4283.
-
Expression of a zinc finger gene in HTLV-I- and HTLV-II-transformed cells.
Wright JJ, Gunter KC, Mitsuya H, Irving SG, Kelly K, Siebenlist U.
Science 1990 May; 248(4955):588.
-
A gene coding for a zinc finger protein is induced during 12-O-tetradecanoylphorbol-13-acetate-stimulated HL-60 cell differentiation.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com