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Last updated: 2017/11/19
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EGR1 polyclonal antibody

  • Catalog # : PAB10007
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of EGR1.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 94-108 of human EGR1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:4000-1:16000)
    Western Blot (1:500-1:3000)
    Immunohistochemistry (2-20 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using EGR1 polyclonal antibody (Cat # PAB10007) shows detectionof a predominant band at ~58 KDa correspond-ing to EGR1 present in mouse embryonic fibroblast whole cell lysate (arrowhead).
    Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.
    After blocking the membrane was probed with the primary antibody diluted to 1:1,500.
    Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of EGR1 polyclonal antibody (Cat # PAB10007) was used at a 10 ug/mL to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides.
    Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining.
    This image shows EGR1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1958
  • Gene Name:
  • EGR1
  • Gene Alias:
  • AT225,G0S30,KROX-24,NGFI-A,TIS8,ZIF-268,ZNF225
  • Gene Description:
  • early growth response 1
  • Gene Summary:
  • The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq
  • Other Designations:
  • early growth response protein 1,nerve growth factor-induced protein A,transcription factor ETR103,zinc finger protein 225
  • Gene Pathway
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