Western blot using EGR1 polyclonal antibody (Cat # PAB10007) shows detectionof a predominant band at ~58 KDa correspond-ing to EGR1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500. Reaction occurred 2h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.
Immunohistochemistry of EGR1 polyclonal antibody (Cat # PAB10007) was used at a 10 ug/mL to detect nuclear and cytoplasmic signal with low background staining in a variety of tissues including multi-human, multi-brain and multi-cancer slides. Within the multi-tumor block, the antibody showed variable levels of nuclear and cytoplasmic staining between individual tumors, with some tumors showing moderate staining. This image shows EGR1 staining of human ovarian carcinoma. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, Life Span Biosciences, Seattle, WA.
The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentitation and mitogenesis. Studies suggest this is a cancer suppresor gene. [provided by RefSeq
early growth response protein 1,nerve growth factor-induced protein A,transcription factor ETR103,zinc finger protein 225