Rabbit polyclonal antibody raised against synthetic phosphopeptide of ECT2.
Synthetic phosphopeptide corresponding to residues surrounding T790 of human ECT2.
Chicken, Chimpanzee, Dog, Human, Mouse, Rat, Zebra fish
Reactivity occurs against human ECT2 protein at the pT790 residue. By ELISA This antibody is specific to the phosphorylated form of the protein. Reactivity with non-phosphorylated human ECT2 is minimal by ELISA, but western blot shows specific staining of interphase cell lysates, which are predicted to contain non-phosphorylated ECT2.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
ELISA (1:10000-1:40000) Western Blot (1:1000-1:4000) The optimal working dilution should be determined by the end user.
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using ECT2 (phospho T790) polyclonal antibody (Cat # PAB10006) showsdetection of endogenous phospho-ECT2 (arrowhead) present in cell lysates from interphase (Lane 1) and mitotic (Lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA resultsusing both phosphorylated and non-phosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 1,000. Personal Communication, Toru Miki,CCR-NCI, Bethesda, MD.
The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. [provided by RefSeq
epithelial cell transforming sequence 2 oncogene protein