DAXX polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DAXX.
Immunogen
A synthetic peptide corresponding to amino acids 261-274 of human DAXX.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:7000-1:45000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using DAXX polyclonal antibody (Cat # PAB10002) shows detection of DAXX in RWPE-1 cell extracts.
The membrane was probed with the primary antibody diluted to 1 : 200, and further probed with peroxidase conjugated anti-Rabbit IgG at a 1 : 25,000 dilution.
Personal Communication, Jie LIU, CCR-NCI, Bethesda, MD.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — DAXX
Entrez GeneID
1616Protein Accession#
NP_001341;Q9UER7Gene Name
DAXX
Gene Alias
BING2, DAP6, EAP1, MGC126245, MGC126246
Gene Description
death-domain associated protein
Omim ID
603186Gene Ontology
HyperlinkGene Summary
This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
CENP-C binding protein|ETS1-associated protein 1|Fas-binding protein|OTTHUMP00000029289|OTTHUMP00000029290|death-associated protein 6
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of Daxx interacting with p73, one of the p53 family, and its regulation of p53 activity by competitive interaction with PML.
Kim EJ, Park JS, Um SJ.
Nucleic Acids Research 2003 Sep; 31(18):5356.
Application:IP, Human, Saos2 cells.
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Daxx and histone deacetylase II associate with chromatin through an interaction with core histones and the chromatin-associated protein Dek.
Hollenbach AD, McPherson CJ, Mientjes EJ, Iyengar R, Grosveld G.
Journal of Cell science 2002 Aug; 115(Pt 16):3319.
Application:IP, WB-Re, Human, 293T cells.
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Identification of Daxx interacting with p73, one of the p53 family, and its regulation of p53 activity by competitive interaction with PML.
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