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Last updated: 2017/3/26
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CDK9 (phospho T29) polyclonal antibody

  • Catalog # : PAB10000
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDK9.
  • Immunogen:
  • Synthetic phosphopeptide corresponding to residues surrounding T29 of human CDK9.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse, Rat
  • Specificity:
  • This antibody is specific to human CDK9 protein phosphorylated at T29.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:5000-1:24000)
    Western Blot (1:200-1:2000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • Western blot using CDK9 (phospho T29) polyclonal antibody (Cat # PAB10000) shows detection of phospho-CDK9.
    100 ng of purified P-TEFb, which contains CDK9 and its regulatory cyclin T1 subunit, was incubated with ATP in the presence or absence of Brd4, a protein known to induce CDK9 phosphorylation at T29.
    The primary antibody was used at a 1:1000 dilution.
    Personal Communication, J. Brady, NCI, Bethesda, MD.
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Recombinant protein)
  • Western Blot (Recombinant protein)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1025
  • Protein Accession#:
  • NP_001252;P50750
  • Gene Name:
  • CDK9
  • Gene Alias:
  • C-2k,CDC2L4,CTK1,PITALRE,TAK
  • Gene Description:
  • cyclin-dependent kinase 9
  • Gene Summary:
  • The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq
  • Other Designations:
  • CDC2-related kinase,OTTHUMP00000022198,cell division protein kinase 9,serine/threonine protein kinase PITALRE
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