SUMO1 polyclonal antibody
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More Files
- More Functions
Western Blot
Western blot detection of recombinant SUMO1 protein using sheep SUMO1 polyclonal antibody (Cat # PAB0765) at a dilution of 1 : 8000. The protein sample was 1 uL of E.Coli cell lysate containing approximately 50 ng of recombinant SUMO1 protein. This antibody recognises a major band of approximately 17 KDa corresponding to SUMO1, as well as a smaller minor band of 8 KDa.
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Specification
Product Description
Sheep polyclonal antibody raised against synthetic peptide of SUMO1.
Immunogen
A synthetic peptide (conjugated diphtheria toxoid) corresponding to amino acids 6-21 of human SUMO1.
Sequence
AKPSTEDLGDKKEGEY
Host
Sheep
Reactivity
Human, Rat
Specificity
This antiserum recognizes human SUMO-1 and not ubiquitin.
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:2000-1:4000)
Western Blot (1:4000-1:8000)
The optimal dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot
Western blot detection of recombinant SUMO1 protein using sheep SUMO1 polyclonal antibody (Cat # PAB0765) at a dilution of 1 : 8000. The protein sample was 1 uL of E.Coli cell lysate containing approximately 50 ng of recombinant SUMO1 protein. This antibody recognises a major band of approximately 17 KDa corresponding to SUMO1, as well as a smaller minor band of 8 KDa.Immunohistochemistry (Frozen sections)
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Gene Info — SUMO1
Entrez GeneID
7341Protein Accession#
P63165Gene Name
SUMO1
Gene Alias
DAP-1, GMP1, OFC10, PIC1, SENP2, SMT3, SMT3C, SMT3H3, SUMO-1, UBL1
Gene Description
SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae)
Omim ID
601912Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last four amino acids of the carboxy-terminus have been cleaved off. Several pseudogenes have been reported for this gene. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq
Other Designations
GAP modifying protein 1|SMT3 suppressor of mif two 3 homolog 1|sentrin|ubiquitin-like 1 (sentrin)
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Interactome
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Disease
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