Sheep polyclonal antibody raised against synthetic peptide of Gja5.
A synthetic peptide (conjugated diphtheria toxoid) corresponding to amino acids 253-269 of rat Gja5.
Theoretical MW (kDa):
Western blotting of cell membarnes of COS cells transiently transfected with cDNA encoding Connexin-40 shows two bands of molecular weight 38 and 40 KDa which are abolished by preincubation with the immunising peptide. No such bands are detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-37, Connexin-43 or Connexin-45.
Immunofluorescence (1:10000-1:50000) Western Blot (1:10000-1:50000) The optimal working dilution should be determined by the end user.
Lyophilized from PBS
Store at 4°C on dry atmosphere. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.