Sheep polyclonal antibody raised against synthetic peptide of Gja4.
A synthetic peptide (conjugated diphtheria toxoid) corresponding to amino acids 265-280 of rat Gja4.
Theoretical MW (kDa):
Western blotting of cell membarnes of COS cells transiently transfected with cDNA encoding Connexin-37 shows two bands of molecular weight 36 and 37 KDa which are abolished by preincubation with the immunising peptide. No such bands are detected in western blots of membranes from non-transfected cells or cells transfected with cDNA encoding Connexin-40, Connexin-43 or Connexin-45.
Immunofluorescence (1:1000-1:5000) Western Blot (1:5000-1:10000) The optimal working dilution should be determined by the end user.
Lyophilized from PBS
Store at 4°C on dry atmosphere. After reconstitution with deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.