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Last updated: 2017/6/25
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ATG3 polyclonal antibody

  • Catalog # : PAB0740
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ATG3.
  • Immunogen:
  • A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to N-terminus of human ATG3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Rat
  • Form:
  • Lyophilized
  • Recommend Usage:
  • Immunohistochemistry (1:50-1:3000)
    Immunofluorescence (1:50-1:3000)
    Western Blot (1:50-1:3000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot under reducing conditions on 293 cell lysate using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 50.
  • Immunohistochemistry (Frozen sections)
  • Immunofluorescence
  • Immunofluorescence
  • Confocal microscopy on immunofluorescently detected ATG3 in cytospin - isolated human white blood cells using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG3 appear in red. The cells were counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line using rabbit ATG3 polyclonal antibody (Cat # PAB0740) .
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Frozen sections)
  • Gene Information
  • Protein Accession#:
  • Q9NT62;Q9CPX6;Q6AZ50;P40344;
  • Gene Name:
  • ATG3
  • Gene Alias:
  • APG3,APG3-LIKE,APG3L,DKFZp564M1178,FLJ22125,MGC15201,PC3-96
  • Gene Description:
  • ATG3 autophagy related 3 homolog (S. cerevisiae)
  • Gene Summary:
  • Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM
  • Other Designations:
  • 2610016C12Rik,APG3 autophagy 3-like,Apg3p,autophagy Apg3p/Aut1p-like
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