Western blot under reducing conditions on 293 cell lysate using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 50.
Immunohistochemistry (Frozen sections)
Confocal microscopy on immunofluorescently detected ATG3 in cytospin - isolated human white blood cells using rabbit ATG3 polyclonal antibody (Cat # PAB0740) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG3 appear in red. The cells were counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hour, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X. No staining was observed using the Pre - immunisation serum. No staining was observed in HL-60 cell line using rabbit ATG3 polyclonal antibody (Cat # PAB0740) .
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM