ATG16L1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot (non - reducing) on Jurkat cell lysate using rabbit ATG16L1 polyclonal antibody (Cat # PAB0739) .
Immunofluorescence
Confocal microscopy on immunofluorescently detected cytospinned HL-60 cell line. HL-60 cells were fixed with acetone 90 seconds ; primary antibody was diluted 1 : 100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) for 30 minutes. Eosinophils seem to be stained using rabbit ATG16L1 polyclonal antibody (Cat # PAB0739) .
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATG16L1.
Immunogen
A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to N-terminus of human ATG16L1.
Host
Rabbit
Reactivity
Human, Rat
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:100-1:3000)
Immunofluorescence (1:100-1:3000)
Western Blot (1:100-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot (non - reducing) on Jurkat cell lysate using rabbit ATG16L1 polyclonal antibody (Cat # PAB0739) .Immunohistochemistry (Frozen sections)
Immunofluorescence
Confocal microscopy on immunofluorescently detected cytospinned HL-60 cell line. HL-60 cells were fixed with acetone 90 seconds ; primary antibody was diluted 1 : 100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) for 30 minutes. Eosinophils seem to be stained using rabbit ATG16L1 polyclonal antibody (Cat # PAB0739) . -
Gene Info — ATG16L1
Entrez GeneID
55054Protein Accession#
Q676U5Gene Name
ATG16L1
Gene Alias
APG16L, ATG16L, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, WDR30
Gene Description
ATG16 autophagy related 16-like 1 (S. cerevisiae)
Gene Ontology
HyperlinkGene Summary
Autophagy is the major intracellular degradation system delivering cytoplasmic components to lysosomes, and it accounts for degradation of most long-lived proteins and some organelles. Cytoplasmic constituents, including organelles, are sequestered into double-membraned autophagosomes, which subsequently fuse with lysosomes. ATG16L1 is a component of a large protein complex essential for autophagy (Mizushima et al., 2003 [PubMed 12665549]).[supplied by OMIM
Other Designations
APG16 autophagy 16-like|APG16L beta|ATG16 autophagy related 16-like protein 1|WD repeat domain 30
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Interactome
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Disease
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