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Last updated: 2016/12/4
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ATG10 polyclonal antibody

  • Catalog # : PAB0732
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of ATG10.
  • Immunogen:
  • A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to C-terminus of human ATG10.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Lyophilized
  • Recommend Usage:
  • Immunohistochemistry (1:100-1:1000)
    Immunofluorescence (1:100-1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • Lyophilized from PBS
  • Storage Instruction:
  • Store at 4°C on dry atmosphere.
    After reconstitution with deionized water, store at -20°C or lower.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Immunohistochemistry (Frozen sections)
  • Immunofluorescence
  • Immunofluorescence
  • Confocal microscopy on immunofluorescently detected ATG10 in cytospin-isolated human white blood cells using rabbit ATG10 polyclonal antibody (Cat # PAB0732) at a dilution of 1 : 100, incubated for 1 hr at room temperature. The cells stained for ATG10 appear in red. The cells were then counter stained with Hoechst Dye (blue colour) . Here, the merged picture is presented. More information on the staining : Fixation : In acetone for 90 seconds. Antibody dilutions : Primary antibody was diluted 1 : 100 and incubated for 1 hr, washed then 3x, and the secondary (Goat anti Rb 1 : 100 alexa 546 conjugate) was incubated for 30 minutes. Magnification 80X.
  • Application Image
  • Immunohistochemistry (Frozen sections)
  • Gene Information
  • Gene Name:
  • ATG10
  • Gene Alias:
  • APG10,APG10L,DKFZp586I0418,FLJ13954,pp12616
  • Gene Description:
  • ATG10 autophagy related 10 homolog (S. cerevisiae)
  • Gene Summary:
  • Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM
  • Other Designations:
  • APG10 autophagy 10-like
  • Related Disease
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