GABARAP polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 100.
Western Blot (Cell lysate)
Western blot under reducing conditions on 293 cell lysate using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 100.
Immunofluorescence
Confocal microscopy on immunofluorescently detected GABARAP in cytospin-isolated human white blood cells using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 200, incubated for 1 hr at room temperature. The cells were double - stained for GABARAP appearing in red (A) and MHC class II appearing in green (B) . The cells were counter stained with Hoechst Dye (C, blue colour) . Here, the merged picture is presented.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of GABARAP.
Immunogen
A synthetic peptide (conjugated with Blue Carrier Protein) corresponding to N-terminus of human GABARAP.
Host
Rabbit
Reactivity
Human, Rat
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Western Blot (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot under reducing conditions on Odora (an Olfactory cell line) cell lysate using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 100.Western Blot (Cell lysate)
Western blot under reducing conditions on 293 cell lysate using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 100.Immunohistochemistry (Frozen sections)
Immunofluorescence
Confocal microscopy on immunofluorescently detected GABARAP in cytospin-isolated human white blood cells using rabbit GABARAP polyclonal antibody (Cat # PAB0730) at a dilution of 1 : 200, incubated for 1 hr at room temperature. The cells were double - stained for GABARAP appearing in red (A) and MHC class II appearing in green (B) . The cells were counter stained with Hoechst Dye (C, blue colour) . Here, the merged picture is presented. -
Gene Info — GABARAP
Entrez GeneID
11337Protein Accession#
O95166;Q9DCD6;P60517Gene Name
GABARAP
Gene Alias
FLJ25768, MGC120154, MGC120155, MM46
Gene Description
GABA(A) receptor-associated protein
Omim ID
605125Gene Ontology
HyperlinkGene Summary
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton. [provided by RefSeq
Other Designations
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