ATG5 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot on non - reduced human neutrophils using rabbit ATG5 polyclonal antibody (Cat # PAB0712) at a dilution of 1 : 100 . The detected band has an apparent molecular weight of 49 KDa corresponding to the ATG5-ATG12 complex molecule.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DAB staining of ATG5 in paraffin - embedded human brain section (midfrontal cortex, Alzheimer case) using rabbit ATG5 polyclonal antibody (Cat # PAB0712) .
Immunofluorescence
Confocal microscopy on stained ATG5 using rabbit ATG5 polyclonal antibody (Cat # PAB0712) in paraffin - embedded human brain section (midfrontal cortex, Alzheimer case) . ATG5 appears green. Red staining is p25.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATG5.
Immunogen
A synthetic peptide corresponding to C-terminus of human ATG5.
Host
Rabbit
Reactivity
Human
Form
Lyophilized
Recommend Usage
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Western Blot (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS
Storage Instruction
Store at 4°C on dry atmosphere.
After reconstitution with deionized water, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
Western blot on non - reduced human neutrophils using rabbit ATG5 polyclonal antibody (Cat # PAB0712) at a dilution of 1 : 100 . The detected band has an apparent molecular weight of 49 KDa corresponding to the ATG5-ATG12 complex molecule.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
DAB staining of ATG5 in paraffin - embedded human brain section (midfrontal cortex, Alzheimer case) using rabbit ATG5 polyclonal antibody (Cat # PAB0712) .Immunohistochemistry (Frozen sections)
Immunofluorescence
Confocal microscopy on stained ATG5 using rabbit ATG5 polyclonal antibody (Cat # PAB0712) in paraffin - embedded human brain section (midfrontal cortex, Alzheimer case) . ATG5 appears green. Red staining is p25. -
Gene Info — ATG5
Entrez GeneID
9474Protein Accession#
Q9H1Y0Gene Name
ATG5
Gene Alias
APG5, APG5-LIKE, APG5L, ASP, hAPG5
Gene Description
ATG5 autophagy related 5 homolog (S. cerevisiae)
Omim ID
604261Gene Ontology
HyperlinkGene Summary
S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
Other Designations
APG5 (autophagy 5, S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
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Interactome
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Pathway
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Disease
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Publication Reference
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Enhancement of neutrophil autophagy by an IVIG preparation against multidrug-resistant bacteria as well as drug-sensitive strains.
Itoh H, Matsuo H, Kitamura N, Yamamoto S, Higuchi T, Takematsu H, Kamikubo Y, Kondo T, Yamashita K, Sasada M, Takaori-Kondo A, Adachi S.
Journal of Leukocyte Biology 2015 Jul; 98(1):107.
Application：IF, Human, Neutrophils.
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Enhancement of neutrophil autophagy by an IVIG preparation against multidrug-resistant bacteria as well as drug-sensitive strains.
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