Histone H3 (K9me2) polyclonal antibody
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More Files
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ChIP
ChIP results obtained with the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
ChIP assays were performed using undifferentiated human teratocarcinoma cells (NCCIT), the affinity purified antibody directed against Histone H3 (K9me2) and optimized PCR primer sets for qPCR.
Western Blot (Cell lysate)
Western blot analysis using the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
Western blot was performed using histone extracts from HeLa cells (HeLa NE, 15 ug) and the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.
The location of the protein of interest is indicated with an arrow.
Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the concentration of a primary antibody using a series of dilutions of crude sera, affinity purified Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653) and flow-through in antigen coated wells.
The antigen used in this case is the peptide including the histone modification of interest.
We plotted the absorbance versus antibody dilution to estimate the TITER : 1 : 160,000 for crude serum and 1 : 100,000 for affinity purified Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
Dot Blot
Cross reactivity tests using the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
Dot Blot is performed to test the cross-reactivity of the Histone H3 (K9me2) polyclonal antibody with other histones and other histone modifications.
Other histone modifications include mono- and trimethylation of the same lysine, mono-, di- and trimethylation of adjacent lysines and acetylations of one or more lysines on histone H4.
To determine the cross-reactivity, 1 to 25 pmol of peptide containing the respective histone modifications were spotted on a membrane.
The antibody was used at a dilution of 1 : 10,000.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of histone H3 (dimethylated lysine 9).
Immunogen
A synthetic peptide (conjugated with KLH) containing the dimethylated lysine 9 (or [K9me2]) of human histone H3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP results obtained with the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
ChIP assays were performed using undifferentiated human teratocarcinoma cells (NCCIT), the affinity purified antibody directed against Histone H3 (K9me2) and optimized PCR primer sets for qPCR.Western Blot (Cell lysate)
Western blot analysis using the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
Western blot was performed using histone extracts from HeLa cells (HeLa NE, 15 ug) and the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.
The location of the protein of interest is indicated with an arrow.Enzyme-linked Immunoabsorbent Assay
ELISA is a quantitative method used to determine the concentration of a primary antibody using a series of dilutions of crude sera, affinity purified Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653) and flow-through in antigen coated wells.
The antigen used in this case is the peptide including the histone modification of interest.
We plotted the absorbance versus antibody dilution to estimate the TITER : 1 : 160,000 for crude serum and 1 : 100,000 for affinity purified Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).Dot Blot
Cross reactivity tests using the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
Dot Blot is performed to test the cross-reactivity of the Histone H3 (K9me2) polyclonal antibody with other histones and other histone modifications.
Other histone modifications include mono- and trimethylation of the same lysine, mono-, di- and trimethylation of adjacent lysines and acetylations of one or more lysines on histone H4.
To determine the cross-reactivity, 1 to 25 pmol of peptide containing the respective histone modifications were spotted on a membrane.
The antibody was used at a dilution of 1 : 10,000. -
Publication Reference
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Temporal Regulation of Nuclear Factor One Occupancy by Calcineurin/NFAT Governs a Voltage-Sensitive Developmental Switch in Late Maturing Neurons.
Ding B, Wang W, Selvakumar T, Xi HS, Zhu H, Chow CW, Horton JD, Gronostajski RM, Kilpatrick DL.
Journal of Neuroscience 2013 Feb; 33(7):2860.
Application：WB, Mouse, Mouse cerebellar granule neurons.
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Dynamic regulation of histone lysine methylation by demethylases.
Shi Y, Whetstine JR.
Molecular Cell 2007 Jan; 25(1):1.
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Intra- and inter-nucleosomal protein-DNA interactions of the core histone tail domains in a model system.
Zheng C, Hayes JJ.
The Journal of Biological Chemistry 2003 Apr; 278(26):24217.
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Translating the histone code.
Jenuwein T, Allis CD.
Science 2001 Aug; 293(5532):1074.
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The language of covalent histone modifications.
Strahl BD, Allis CD.
Nature 2000 Jan; 403(6765):41.
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Temporal Regulation of Nuclear Factor One Occupancy by Calcineurin/NFAT Governs a Voltage-Sensitive Developmental Switch in Late Maturing Neurons.
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