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Last updated: 2017/2/19
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Histone H3 (K9me2) polyclonal antibody

  • Catalog # : PAB0653
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of histone H3 (dimethylated lysine 9).
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) containing the dimethylated lysine 9 (or [K9me2]) of human histone H3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% proclin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • ChIP
  • ChIP
  • ChIP results obtained with the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
    ChIP assays were performed using undifferentiated human teratocarcinoma cells (NCCIT), the affinity purified antibody directed against Histone H3 (K9me2) and optimized PCR primer sets for qPCR.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
    Western blot was performed using histone extracts from HeLa cells (HeLa NE, 15 ug) and the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653) at dilution 1 : 1,000 in TBS-Tween + 5% skimmed milk.
    The location of the protein of interest is indicated with an arrow.
  • ELISA
  • ELISA
  • ELISA is a quantitative method used to determine the concentration of a primary antibody using a series of dilutions of crude sera, affinity purified Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653) and flow-through in antigen coated wells.
    The antigen used in this case is the peptide including the histone modification of interest.
    We plotted the absorbance versus antibody dilution to estimate the TITER : 1 : 160,000 for crude serum and 1 : 100,000 for affinity purified Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
  • Dot Blot
  • Dot Blot
  • Cross reactivity tests using the Histone H3 (K9me2) polyclonal antibody (Cat # PAB0653).
    Dot Blot is performed to test the cross-reactivity of the Histone H3 (K9me2) polyclonal antibody with other histones and other histone modifications.
    Other histone modifications include mono- and trimethylation of the same lysine, mono-, di- and trimethylation of adjacent lysines and acetylations of one or more lysines on histone H4.
    To determine the cross-reactivity, 1 to 25 pmol of peptide containing the respective histone modifications were spotted on a membrane.
    The antibody was used at a dilution of 1 : 10,000.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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