Histone H3 (K9ac) polyclonal antibody
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More Files
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ChIP
ChIP results obtained with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651). ChIP assays were performed using U-2 OS cells.
Immunofluorescence
Immunofluorescence with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) .
HeLa cells were stained with antibody directed against modified histone and with DAPI.
Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS.
The fixation step stabilises the morphology of the cells and permeabilizes membranes as well.
(A) Cells were immunofluorescently labelled with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) (diluted 1 : 200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to FITC.
(B) Nuclei were DAPI stained to label specifically the DNA.
In both cases, the nuclei are clearly stained.
Enzyme-linked Immunoabsorbent Assay
ELISA, which stands for Enzyme-Linked Immunosorbent Assay, is a quantitative method used to determine the concentration of a primary antibody in sera by using a series of dilutions of sera in antigen coated wells. The antigen used in this case is the peptide including the histone H3 [acetylated lysin 9]. We plotted the absorbance versus antiserum dilution to estimate the titer of the antibody.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of histone H3 (acetylated lysine 9).
Immunogen
A synthetic peptide (conjugated with KLH) containing the acetylated lysine 9 (or [K9ac]) of human histone H3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.1% BSA, 0.01% thimerosal)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP results obtained with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651). ChIP assays were performed using U-2 OS cells.Immunofluorescence
Immunofluorescence with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) .
HeLa cells were stained with antibody directed against modified histone and with DAPI.
Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS.
The fixation step stabilises the morphology of the cells and permeabilizes membranes as well.
(A) Cells were immunofluorescently labelled with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) (diluted 1 : 200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to FITC.
(B) Nuclei were DAPI stained to label specifically the DNA.
In both cases, the nuclei are clearly stained.Enzyme-linked Immunoabsorbent Assay
ELISA, which stands for Enzyme-Linked Immunosorbent Assay, is a quantitative method used to determine the concentration of a primary antibody in sera by using a series of dilutions of sera in antigen coated wells. The antigen used in this case is the peptide including the histone H3 [acetylated lysin 9]. We plotted the absorbance versus antiserum dilution to estimate the titer of the antibody.Dot Blot (Peptide)
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