ChIP results obtained with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651). ChIP assays were performed using U-2 OS cells.
Immunofluorescence with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) . HeLa cells were stained with antibody directed against modified histone and with DAPI. Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS. The fixation step stabilises the morphology of the cells and permeabilizes membranes as well. (A) Cells were immunofluorescently labelled with the Histone H3 (K9ac) polyclonal antibody (Cat # PAB0651) (diluted 1 : 200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to FITC. (B) Nuclei were DAPI stained to label specifically the DNA. In both cases, the nuclei are clearly stained.
ELISA, which stands for Enzyme-Linked Immunosorbent Assay, is a quantitative method used to determine the concentration of a primary antibody in sera by using a series of dilutions of sera in antigen coated wells. The antigen used in this case is the peptide including the histone H3 [acetylated lysin 9]. We plotted the absorbance versus antiserum dilution to estimate the titer of the antibody.