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Last updated: 2017/12/10
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Histone H3 (K4me3) polyclonal antibody

  • Catalog # : PAB0650
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of histone H3 (trimethylated lysine 4).
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) containing the trimethylated lysine 4 (or [K4me3]) of human histone H3.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Recommend Usage:
  • The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.1% BSA, 0.01% thimerosal)
  • Storage Instruction:
  • Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP results obtained with the Histone H3 (K4me3) polyclonal antibody (Cat # PAB0650). ChIP assays were performed using U-2 OS cells.
  • ChIP on chip
  • ChIP on chip
  • ChIP-on-chip profile using the Histone H3 (K4me3) polyclonal antibody (Cat # PAB0650) . Here above the Histone H3 (K4me3) ChIP-on-chip profile of an ubiquitin-like gene (GenBank AK147046) on chromosome 17 of two different mouse cell lines (ES14 in blue and LF2 in purple) is presented.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence with the Histone H3 (K4me3) polyclonal antibody (Cat # PAB0650).
    HeLa cells were stained with antibody directed against modified histone and with DAPI.
    Cells were methanol fixed for 10 minutes and then blocked with 1% BSA containing PBS.
    The fixation step stabilises the morphology of the cells and permeabilizes membranes as well.
    (A) Cells were immunofluorescently labelled with the Histone H3 (K4me3) polyclonal antibody (Cat # PAB0650) (diluted 1 : 200 and incubated for 1 hour at room temperature) followed by goat anti-rabbit antibody conjugated to FITC.
    (B) Nuclei were DAPI stained to label specifically the DNA.
    In both cases, the nuclei are clearly stained.
  • ELISA is a quantitative method used to determine the concentration of a primary antibody in sera by using a series of dilutions of sera in antigen coated wells. The antigen used in this case is the peptide including the Histone H3 (K4me3) [ trimethylated lysine 4 ]. We plotted the absorbance versus antiserum dilution to estimate the titer of the affinity purified antibody : 1 : 50,000.
  • Dot Blot (Peptide)
  • Application Image
  • Dot Blot (Peptide)
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