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Last updated: 2017/10/22
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MECP2 polyclonal antibody

  • Catalog # : PAB0646
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of human MECP2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MECP2.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Concentration:
  • 1.2 ug/uL
  • Recommend Usage:
  • ChIP (5 ug/ChIP)
    ELISA (1:1000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% ProClin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP results obtained with the MECP2 polyclonal antibody (Cat # PAB0646). ChIP assays were performed using human osteosarcoma (U-2 OS) cells. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis from 40 ug HeLa nuclear extracts using MECP2 polyclonal antibody (Cat # PAB0646) at 1:1000 dilution.
  • ELISA
  • ELISA
  • To determine the titer of the antibody, an ELISA was performed using a serial dilution of MECP2 polyclonal antibody (Cat # PAB0646) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 4204
  • Gene Name:
  • MECP2
  • Gene Alias:
  • AUTSX3,DKFZp686A24160,MRX16,MRX79,MRXS13,MRXSL,PPMX,RTS,RTT
  • Gene Description:
  • methyl CpG binding protein 2 (Rett syndrome)
  • Gene Summary:
  • DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000026021,methyl CpG binding protein 2
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