ChIP results obtained with the Mecp2 polyclonal antibody (Cat # PAB0646). ChIP assays were performed using human osteosarcoma (U-2 OS) cells. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Western Blot (Cell lysate)
Western blot analysis from 40 ug HeLa nuclear extracts using Mecp2 polyclonal antibody (Cat # PAB0646) at 1:1000 dilution.
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Mecp2 polyclonal antibody (Cat # PAB0646) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.