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Last updated: 2017/3/26
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Mecp2 polyclonal antibody

  • Catalog # : PAB0646
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of Mecp2.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to C-terminus of mouse MeCP2.
  • Host:
  • Rabbit
  • Reactivity:
  • Mouse
  • Form:
  • Liquid
  • Purification:
  • Affinity purification
  • Concentration:
  • 1.2 ug/uL
  • Recommend Usage:
  • ChIP (5 ug/ChIP)
    ELISA (1:1000)
    Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.05% sodium azide, 0.05% ProClin 300)
  • Storage Instruction:
  • Store at -20°C. For long term storage store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • ChIP
  • ChIP
  • ChIP results obtained with the Mecp2 polyclonal antibody (Cat # PAB0646). ChIP assays were performed using human osteosarcoma (U-2 OS) cells. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis from 40 ug HeLa nuclear extracts using Mecp2 polyclonal antibody (Cat # PAB0646) at 1:1000 dilution.
  • ELISA
  • ELISA
  • To determine the titer of the antibody, an ELISA was performed using a serial dilution of Mecp2 polyclonal antibody (Cat # PAB0646) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Gene Name:
  • Mecp2
  • Gene Alias:
  • 1500041B07Rik,BB130002,D630021H01Rik,Mbd5,WBP10
  • Gene Description:
  • methyl CpG binding protein 2
  • Other Designations:
  • OTTMUSP00000019241,OTTMUSP00000019680
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