MECP2 polyclonal antibody
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More Files
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ChIP
ChIP results obtained with the MECP2 polyclonal antibody (Cat # PAB0646). ChIP assays were performed using human osteosarcoma (U-2 OS) cells. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
Western Blot (Cell lysate)
Western blot analysis from 40 ug HeLa nuclear extracts using MECP2 polyclonal antibody (Cat # PAB0646) at 1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
To determine the titer of the antibody, an ELISA was performed using a serial dilution of MECP2 polyclonal antibody (Cat # PAB0646) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of human MECP2.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human MECP2.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Concentration
1.2 ug/uL
Recommend Usage
ChIP (5 ug/ChIP)
ELISA (1:1000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% ProClin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
ChIP
ChIP results obtained with the MECP2 polyclonal antibody (Cat # PAB0646). ChIP assays were performed using human osteosarcoma (U-2 OS) cells. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).Western Blot (Cell lysate)
Western blot analysis from 40 ug HeLa nuclear extracts using MECP2 polyclonal antibody (Cat # PAB0646) at 1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
To determine the titer of the antibody, an ELISA was performed using a serial dilution of MECP2 polyclonal antibody (Cat # PAB0646) and the crude serum. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be: 1:32,900. -
Gene Info — MECP2
Entrez GeneID
4204Protein Accession#
P51608Gene Name
MECP2
Gene Alias
AUTSX3, DKFZp686A24160, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT
Gene Description
methyl CpG binding protein 2 (Rett syndrome)
Gene Ontology
HyperlinkGene Summary
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. [provided by RefSeq
Other Designations
OTTHUMP00000026021|methyl CpG binding protein 2
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Interactome
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Disease
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Publication Reference
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Inhibition of Gsk3b Reduces Nfkb1 Signaling and Rescues Synaptic Activity to Improve the Rett Syndrome Phenotype in Mecp2-Knockout Mice.
Jorge-Torres OC, Szczesna K, Roa L, Casal C, Gonzalez-Somermeyer L, Soler M, Velasco CD, Martínez-San Segundo P, Petazzi P, Sáez MA, Delgado-Morales R, Fourcade S, Pujol A, Huertas D, Llobet A, Guil S, Esteller M.
Cell Reports 2018 May; 23(6):1665.
Application：WB-Ti, Human, Mouse, Brains from human, Mouse.
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Inhibition of Gsk3b Reduces Nfkb1 Signaling and Rescues Synaptic Activity to Improve the Rett Syndrome Phenotype in Mecp2-Knockout Mice.
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