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Last updated: 2016/12/11
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WEE1 (phospho S123) polyclonal antibody

  • Catalog # : PAB0632
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of WEE1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S123 of human WEE1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Immunohistochemistry (1:50-100)
    Dot Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • WEE1 (phospho S123) polyclonal antibody (Cat # PAB0632). Western blot analysis of WEE1 expression in human kidney.
  • PDF DownloadProtocol Download
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with WEE1 (phospho S123) polyclonal antibody (Cat # PAB0632) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of WEE1 (phospho S123) polyclonal antibody (Cat # PAB0632) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 7465
  • Protein Accession#:
  • NP_003381;P30291
  • Gene Name:
  • WEE1
  • Gene Alias:
  • DKFZp686I18166,FLJ16446,WEE1A,WEE1hu
  • Gene Description:
  • WEE1 homolog (S. pombe)
  • Gene Summary:
  • This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq
  • Other Designations:
  • WEE1+ homolog,wee1 tyrosine kinase,wee1-like protein kinase
  • Interactome
  • Interactome
  • Gene Pathway
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