STAT5A (phospho S726) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of STAT5A (phospho S726) polyclonal antibody (Cat # PAB0620) in CEM cell lysate (35 ug/lane). Phospho-STAT5A (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with STAT5A (phospho S726) polyclonal antibody (Cat # PAB0620) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of STAT5A.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S726 of human STAT5A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of STAT5A (phospho S726) polyclonal antibody (Cat # PAB0620) in CEM cell lysate (35 ug/lane). Phospho-STAT5A (arrow) was detected using the purified polyclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with STAT5A (phospho S726) polyclonal antibody (Cat # PAB0620) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.Dot Blot (Peptide)
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Gene Info — STAT5A
Entrez GeneID
6776Protein Accession#
NP_003143;P42229Gene Name
STAT5A
Gene Alias
MGF, STAT5
Gene Description
signal transducer and activator of transcription 5A
Omim ID
601511Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq
Other Designations
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Publication Reference
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{beta}1 Integrin and IL-3R coordinately regulate STAT5 activation and anchorage-dependent proliferation.
Defilippi P, Rosso A, Dentelli P, Calvi C, Garbarino G, Tarone G, Pegoraro L, Brizzi MF.
The Journal of Cell Biology 2005 Mar; 168(7):1099.
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Suppressor of cytokine signaling 7 inhibits prolactin, growth hormone, and leptin signaling by interacting with STAT5 or STAT3 and attenuating their nuclear translocation.
Martens N, Uzan G, Wery M, Hooghe R, Hooghe-Peters EL, Gertler A.
The Journal of Biological Chemistry 2005 Apr; 280(14):13817.
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Physical and functional interactions between STAP-2/BKS and STAT5.
Sekine Y, Yamamoto T, Yumioka T, Sugiyama K, Tsuji S, Oritani K, Shimoda K, Minoguchi M, Yoshimura A, Matsuda T.
The Journal of Biological Chemistry 2005 Mar; 280(9):8188.
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{beta}1 Integrin and IL-3R coordinately regulate STAT5 activation and anchorage-dependent proliferation.
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