FG Pancreatic Carcinoma Cell stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). SRC (phospho Y215) polyclonal antibody (Cat # PAB0612) was diluted in 1% BSA/TBST and incubated Overnight at 4 °C. After washing 3x 5 min. with TBST the blots were incubated with Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq