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Last updated: 2017/8/20
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SRC (phospho Y215) polyclonal antibody

  • Catalog # : PAB0612
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of SRC.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y215 of human SRC.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • FG Pancreatic Carcinoma Cell stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x).
    SRC (phospho Y215) polyclonal antibody (Cat # PAB0612) was diluted in 1% BSA/TBST and incubated Overnight at 4 °C.
    After washing 3x 5 min. with TBST the blots were incubated with Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at room temperature.
    The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.
    Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 6714
  • Protein Accession#:
  • NP_005408;P12931
  • Gene Name:
  • SRC
  • Gene Alias:
  • ASV,SRC1,c-SRC,p60-Src
  • Gene Description:
  • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
  • Gene Summary:
  • This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000174476,OTTHUMP00000174477,proto-oncogene tyrosine-protein kinase SRC,protooncogene SRC, Rous sarcoma,tyrosine kinase pp60c-src,tyrosine-protein kinase SRC-1
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