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Last updated: 2016/10/23
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PKMYT1 (phospho T495) polyclonal antibody

  • Catalog # : PAB0542
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of PKMYT1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T495 of human PKMYT1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Dot Blot (1:500)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) is used in Western blot to detect Phospho-PKMYT1-T495 in HL-60 (left) and SK-BR-3 (right) cell lysates
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9088
  • Protein Accession#:
  • NP_004194;Q99640
  • Gene Name:
  • PKMYT1
  • Gene Alias:
  • DKFZp547K1610,FLJ20093,MYT1
  • Gene Description:
  • protein kinase, membrane associated tyrosine/threonine 1
  • Gene Summary:
  • The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
  • Other Designations:
  • membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase,protein kinase Myt1
  • Interactome
  • Interactome
  • Gene Pathway
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