Formalin-fixed and paraffin-embedded human cancer tissue reacted with PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Dot Blot (Peptide)
Dot blot analysis of PKMYT1 (phospho T495) polyclonal antibody (Cat # PAB0542) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase preferentially phosphorylates and inactivates cell division cycle 2 protein (CDC2), and thus negatively regulates cell cycle G2/M transition. This kinase is associated with the membrane throughout the cell cycle. Its activity is highly regulated during the cell cycle. Protein kinases AKT1/PKB and PLK (Polo-like kinase) have been shown to phosphorylate and regulate the activity of this kinase. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq
membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase,protein kinase Myt1