MAP2K1 (phospho S218/S222) polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAP2K1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S218/222 of human MAP2K1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Dot Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAP2K1 (phospho S218/222) polyclonal antibody (Cat # PAB0529) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.Dot Blot (Peptide)
Dot blot analysis of MAP2K1 (phospho S218/S222) polyclonal antibody (Cat # PAB0529) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. -
Gene Info — MAP2K1
Entrez GeneID
5604Protein Accession#
NP_002746;Q02750Gene Name
MAP2K1
Gene Alias
MAPKK1, MEK1, MKK1, PRKMK1
Gene Description
mitogen-activated protein kinase kinase 1
Omim ID
176872Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq
Other Designations
protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)
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Interactome
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Pathway
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Disease
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Publication Reference
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Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villen J, Li J, Cohn MA, Cantley LC, Gygi SP.
PNAS 2004 Aug; 101(33):12130.
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Molecular cross-talk between MEK1/2 and mTOR signaling during recovery of 293 cells from hypertonic stress.
Naegele S, Morley SJ.
The Journal of Biological Chemistry 2004 Oct; 279(44):46023.
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MEK1 and MEK2, different regulators of the G1/S transition.
Ussar S, Voss T.
The Journal of Biological Chemistry 2004 Oct; 279(42):43861.
Application:WB, Human, HCT-116 cellls.
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Large-scale characterization of HeLa cell nuclear phosphoproteins.
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