MAPKAPK5 (phospho S93) polyclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Tissue lysate)
The MAPKAPK5 (phospho S93) polyclonal antibody (Cat # PAB0520) is used in Western blot to detect Phospho-MAPKAPK5-S93 in mouse heart tissue lysate. PAD4 (arrow) was detected using the purified polyclonal antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAPKAPK5 (phospho S93) polyclonal antibody (Cat # PAB0520) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
-
Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of MAPKAPK5.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S93 of human MAPKAPK5.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Tissue lysate)
The MAPKAPK5 (phospho S93) polyclonal antibody (Cat # PAB0520) is used in Western blot to detect Phospho-MAPKAPK5-S93 in mouse heart tissue lysate. PAD4 (arrow) was detected using the purified polyclonal antibody.Western Blot (Cell lysate)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with MAPKAPK5 (phospho S93) polyclonal antibody (Cat # PAB0520) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — MAPKAPK5
Entrez GeneID
8550Protein Accession#
NP_620777;Q8IW41Gene Name
MAPKAPK5
Gene Alias
PRAK
Gene Description
mitogen-activated protein kinase-activated protein kinase 5
Omim ID
606723Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq
Other Designations
p38-regulated/activated protein kinase
-
Interactome
-
Pathway
-
Publication Reference
-
PRAK, a novel protein kinase regulated by the p38 MAP kinase.
New L, Jiang Y, Zhao M, Liu K, Zhu W, Flood LJ, Kato Y, Parry GC, Han J.
The EMBO Journal 1998 Jun; 17(12):3372.
-
MAPKAPK5, a novel mitogen-activated protein kinase (MAPK)-activated protein kinase, is a substrate of the extracellular-regulated kinase (ERK) and p38 kinase.
Ni H, Wang XS, Diener K, Yao Z.
Biochemical and Biophysical Research Communications 1998 Feb; 243(2):492.
-
PRAK, a novel protein kinase regulated by the p38 MAP kinase.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com