KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) is used in Western blot to detect Phospho-KDR-Y996 in HMVEC cell lysate. Endothelial cells were stimulated with 50 ug/mL VEGF for 5min; 20 ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) diluted in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with KDR (phospho Y996) polyclonal antibody (Cat # PAB0505) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Vascular endothelial growth factor (VEGF) is a major growth factor for endothelial cells. This gene encodes one of the two receptors of the VEGF. This receptor, known as kinase insert domain receptor, is a type III receptor tyrosine kinase. It functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. The signalling and trafficking of this receptor are regulated by multiple factors, including Rab GTPase, P2Y purine nucleotide receptor, integrin alphaVbeta3, T-cell protein tyrosine phosphatase, etc.. Mutations of this gene are implicated in infantile capillary hemangiomas. [provided by RefSeq