ERBB4 (phospho Y1162) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
ERBB4 (phospho Y1162) polyclonal antibody (Cat # PAB0486) was used to detect Phospho-ERBB4-Y1162 in FG pancreatic carcinoma cell lysate. FG pancreatic carcinoma cells treated with or without EGF (50 ng/ml) for 15 min ERBB4 (phospho Y1162) polyclonal antibody (Cat # PAB0486) was used with 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ERBB4 (phospho Y1162) polyclonal antibody (Cat # PAB0486) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERBB4.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y1162 of human ERBB4.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
ERBB4 (phospho Y1162) polyclonal antibody (Cat # PAB0486) was used to detect Phospho-ERBB4-Y1162 in FG pancreatic carcinoma cell lysate. FG pancreatic carcinoma cells treated with or without EGF (50 ng/ml) for 15 min ERBB4 (phospho Y1162) polyclonal antibody (Cat # PAB0486) was used with 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with ERBB4 (phospho Y1162) polyclonal antibody (Cat # PAB0486) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — ERBB4
Entrez GeneID
2066Protein Accession#
NP_005226;Q15303Gene Name
ERBB4
Gene Alias
HER4, MGC138404, p180erbB4
Gene Description
v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian)
Omim ID
600543Gene Ontology
HyperlinkGene Summary
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq
Other Designations
avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4|receptor tyrosine-protein kinase erbB-4|tyrosine kinase-type cell surface receptor HER4|v-erb-a avian erythroblastic leukemia viral oncogene homolog-like 4|v-erb-a erythroblastic leukemia v
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Interactome
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Disease
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Publication Reference
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Changes in the expression and cytological localization of betacellulin and its receptors (ErbB-1 and ErbB-4) in the trophoblasts in human placenta over the course of pregnancy.
Tanimura K, Nakago S, Murakoshi H, Takekida S, Moriyama T, Matsuo H, Hashimoto K, Maruo T.
European Journal of Endocrinology 2004 Jul; 151(1):93.
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Cell surface expression of epidermal growth factor receptor and Her-2 with nuclear expression of Her-4 in primary osteosarcoma.
Hughes DP, Thomas DG, Giordano TJ, Baker LH, McDonagh KT.
Cancer Research 2004 Mar; 64(6):2047.
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Ectodomain cleavage of ErbB-4: characterization of the cleavage site and m80 fragment.
Cheng QC, Tikhomirov O, Zhou W, Carpenter G.
The Journal of Biological Chemistry 2003 Oct; 278(40):38421.
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Changes in the expression and cytological localization of betacellulin and its receptors (ErbB-1 and ErbB-4) in the trophoblasts in human placenta over the course of pregnancy.
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