EP300 (phospho S89) polyclonal antibody
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More Files
- More Functions
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EP300 (phospho S89) polyclonal antibody (Cat # PAB0461) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
Dot Blot (Peptide)
Dot blot analysis of EP300 (phospho S89) polyclonal antibody (Cat # PAB0461) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of EP300.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S89 of human EP300.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Dot Blot (1:500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with EP300 (phospho S89) polyclonal antibody (Cat # PAB0461) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.Dot Blot (Peptide)
Dot blot analysis of EP300 (phospho S89) polyclonal antibody (Cat # PAB0461) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. -
Gene Info — EP300
Entrez GeneID
2033Protein Accession#
NP_001420;Q09472Gene Name
EP300
Gene Alias
KAT3B, p300
Gene Description
E1A binding protein p300
Gene Ontology
HyperlinkGene Summary
This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq
Other Designations
E1A-associated protein p300|E1A-binding protein, 300kD|OTTHUMP00000028668|histone acetyltransferase p300
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Interactome
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Pathway
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Disease
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Publication Reference
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Interferon regulatory factor 1 binding to p300 stimulates DNA-dependent acetylation of p53.
Dornan D, Eckert M, Wallace M, Shimizu H, Ramsay E, Hupp TR, Ball KL.
Molecular and Cellular Biochemistry 2004 Nov; 24(22):10083.
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The N-terminal interferon-binding domain (IBiD) homology domain of p300 binds to peptides with homology to the p53 transactivation domain.
Finlan L, Hupp TR.
The Journal of Biological Chemistry 2004 Nov; 279(47):49395.
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Transforming growth factor-beta stimulates p300-dependent RUNX3 acetylation, which inhibits ubiquitination-mediated degradation.
Jin YH, Jeon EJ, Li QL, Lee YH, Choi JK, Kim WJ, Lee KY, Bae SC.
The Journal of Biological Chemistry 2004 Jul; 279(28):29409.
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Interferon regulatory factor 1 binding to p300 stimulates DNA-dependent acetylation of p53.
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