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Last updated: 2016/12/4
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JUN (phospho S63) polyclonal antibody

  • Catalog # : PAB0443
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of JUN.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S63 of human JUN.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Dot Blot (1:500)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • Western blot analysis of JUN (phospho S63) polyclonal antibody (Cat # PAB0443) in mouse kidney tissue lysate (35 ug/lane). Phospho-cJun-pS63 (arrow) was detected using the purified polyclonal antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human cancer tissue reacted with JUN (phospho S63) polyclonal antibody (Cat # PAB0443) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of JUN (phospho S63) polyclonal antibody (Cat # PAB0443) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 3725
  • Protein Accession#:
  • NP_002219;P05412
  • Gene Name:
  • JUN
  • Gene Alias:
  • AP-1,AP1,c-Jun
  • Gene Description:
  • jun oncogene
  • Gene Summary:
  • This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq
  • Other Designations:
  • Jun activation domain binding protein,OTTHUMP00000010036,activator protein 1,enhancer-binding protein AP1,v-jun avian sarcoma virus 17 oncogene homolog,v-jun sarcoma virus 17 oncogene homolog
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