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Last updated: 2017/9/17
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CHEK1 (phospho S317) polyclonal antibody

  • Catalog # : PAB0441
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S317 of human CHEK1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    Dot Blot (1:500)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The CHEK1 (phospho S317) polyclonal antibody (Cat # PAB0441) is used in Western blot for detection in, from left to right, Jurkat, A-2058, Hela, and HL-60 cell lysates.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB0441) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • Dot Blot (Peptide)
  • Dot Blot (Peptide)
  • Dot blot analysis of CHEK1 (phospho S317) polyclonal antibody (Cat # PAB0441) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed.
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1111
  • Protein Accession#:
  • NP_001265;O14757
  • Gene Name:
  • CHEK1
  • Gene Alias:
  • CHK1
  • Gene Description:
  • CHK1 checkpoint homolog (S. pombe)
  • Other Designations:
  • CHK1 checkpoint homolog,Checkpoint, S. pombe, homolog of, 1
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