CHEK1 (phospho S317) polyclonal antibody
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More Files
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK1.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S317 of human CHEK1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
Dot Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
The CHEK1 (phospho S317) polyclonal antibody (Cat # PAB0441) is used in Western blot for detection in, from left to right, Jurkat, A-2058, Hela, and HL-60 cell lysates.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CHEK1 (phospho S317) polyclonal antibody (Cat # PAB0441) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.Dot Blot (Peptide)
Dot blot analysis of CHEK1 (phospho S317) polyclonal antibody (Cat # PAB0441) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. -
Gene Info — CHEK1
Entrez GeneID
1111Protein Accession#
NP_001265;O14757Gene Name
CHEK1
Gene Alias
CHK1
Gene Description
CHK1 checkpoint homolog (S. pombe)
Omim ID
603078Gene Ontology
HyperlinkGene Summary
S. pombe
Other Designations
CHK1 checkpoint homolog|Checkpoint, S. pombe, homolog of, 1
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1.
Xu X, Lee J, Stern DF.
The Journal of Biological Chemistry 2004 Aug; 279(33):34091.
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Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains.
Ng CP, Lee HC, Ho CW, Arooz T, Siu WY, Lau A, Poon RY.
The Journal of Biological Chemistry 2004 Mar; 279(10):8808.
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Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1.
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