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Last updated: 2017/6/25
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CHEK1 (phospho S280) polyclonal antibody

  • Catalog # : PAB0440
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of CHEK1.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S280of human CHEK1.
  • Host:
  • Rabbit
  • Reactivity:
  • Human, Mouse
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • The CHEK1 (phospho S280) polyclonal antibody (Cat # PAB0440) is used in Western blot to detect Phospho-CHK1-S280 in mouse brain tissue lysate.
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The CHEK1 (phospho S280) polyclonal antibody (Cat # PAB0440) is used in Western blot to detect Phospho-CHK1-S280 in, from left to right, A2058, Ramos, Jurkat, HL-60, and Hela cell lysates.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CHEK1 (phospho S280) polyclonal antibody (Cat # PAB0440) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 1111
  • Protein Accession#:
  • NP_001265;O14757
  • Gene Name:
  • CHEK1
  • Gene Alias:
  • CHK1
  • Gene Description:
  • CHK1 checkpoint homolog (S. pombe)
  • Other Designations:
  • CHK1 checkpoint homolog,Checkpoint, S. pombe, homolog of, 1
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