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Last updated: 2016/11/27
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CDC25B (phospho S187) polyclonal antibody

  • Catalog # : PAB0432
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC25B.
  • Immunogen:
  • Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S187 of human CDC25B.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • Western Blot (1:1000)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • 2.
  • Nuclear export signal in CDC25B.
    Uchida S, Ohtsubo M, Shimura M, Hirata M, Nakagama H, Matsunaga T, Yoshida M, Ishizaka Y, Yamashita K.Biochem Biophys Res Commun. 2004 Mar 26;316(1):226-32.
  • Applications
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • CDC25B (phospho S187) polyclonal antibody (Cat # PAB0432). Western blot analysis of CDC25B expression in human kidney.
  • PDF DownloadProtocol Download
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • The CDC25B (phospho S187) polyclonal antibody (Cat # PAB0432) is used in Western blot to detect Phospho-CDC25B-S187 in SK-BR-3 cell lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDC25B (phospho S187) polyclonal antibody (Cat # PAB0432) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • Application Image
  • Western Blot (Tissue lysate)
  • Western Blot (Tissue lysate)
  • enlarge
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
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  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 994
  • Protein Accession#:
  • NP_068660;P30305
  • Gene Name:
  • CDC25B
  • Gene Alias:
  • -
  • Gene Description:
  • cell division cycle 25 homolog B (S. pombe)
  • Gene Summary:
  • CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000030137,OTTHUMP00000030138,OTTHUMP00000030139,cell division cycle 25B
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