CDC25B (phospho S187) polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
CDC25B (phospho S187) polyclonal antibody (Cat # PAB0432). Western blot analysis of CDC25B expression in human kidney.
Western Blot (Cell lysate)
The CDC25B (phospho S187) polyclonal antibody (Cat # PAB0432) is used in Western blot to detect Phospho-CDC25B-S187 in SK-BR-3 cell lysate
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDC25B (phospho S187) polyclonal antibody (Cat # PAB0432) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CDC25B.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S187 of human CDC25B.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
CDC25B (phospho S187) polyclonal antibody (Cat # PAB0432). Western blot analysis of CDC25B expression in human kidney.Western Blot (Cell lysate)
The CDC25B (phospho S187) polyclonal antibody (Cat # PAB0432) is used in Western blot to detect Phospho-CDC25B-S187 in SK-BR-3 cell lysateImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with CDC25B (phospho S187) polyclonal antibody (Cat # PAB0432) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma. -
Gene Info — CDC25B
Entrez GeneID
994Protein Accession#
NP_068660;P30305Gene Name
CDC25B
Gene Alias
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Gene Description
cell division cycle 25 homolog B (S. pombe)
Omim ID
116949Gene Ontology
HyperlinkGene Summary
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq
Other Designations
OTTHUMP00000030137|OTTHUMP00000030138|OTTHUMP00000030139|cell division cycle 25B
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Interactome
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Pathway
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Disease
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Publication Reference
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Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
Ito Y, Yoshida H, Matsuzuka F, Matsuura N, Nakamura Y, Nakamine H, Kakudo K, Kuma K, Miyauchi A.
International Journal of Molecular Medicine 2004 Mar; 13(3):431.
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Nuclear export signal in CDC25B.
Uchida S, Ohtsubo M, Shimura M, Hirata M, Nakagama H, Matsunaga T, Yoshida M, Ishizaka Y, Yamashita K.
Biochemical and Biophysical Research Communications 2004 Mar; 316(1):226.
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Coordinate expression of Cdc25B and ER-alpha is frequent in low-grade endometrioid endometrial carcinoma but uncommon in high-grade endometrioid and nonendometrioid carcinomas.
Wu W, Slomovitz BM, Celestino J, Chung L, Thornton A, Lu KH.
Cancer Research 2003 Oct; 63(19):6195.
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Cdc25A and cdc25B expression in malignant lymphoma of the thyroid: correlation with histological subtypes and cell proliferation.
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