CASP6 (phospho S257) polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of CASP6.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S257 of human CASP6.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of CASP6 (phospho S257) polyclonal antibody (Cat # PAB0423) in mouse liver lysates (35 ug/lane). CASP6-pS257 (arrow) was detected using the purified Pab.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with CASP6 (phospho S257) polyclonal antibody (Cat # PAB0423) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma. -
Gene Info — CASP6
Entrez GeneID
839Protein Accession#
NP_001217;P55212Gene Name
CASP6
Gene Alias
MCH2
Gene Description
caspase 6, apoptosis-related cysteine peptidase
Omim ID
601532Gene Ontology
HyperlinkGene Summary
This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade. Alternative splicing of this gene results in two transcript variants that encode different isoforms. [provided by RefSeq
Other Designations
apoptotic protease MCH-2|caspase 6|caspase 6, apoptosis-related cysteine protease
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Breaking the connection: caspase 6 disconnects intermediate filament-binding domain of periplakin from its actin-binding N-terminal region.
Kalinin AE, Kalinin AE, Aho M, Uitto J, Aho S.
The Journal of Investigative Dermatology 2005 Jan; 124(1):46.
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Regulation of caspase-6 and FLIP by the AMPK family member ARK5.
Suzuki A, Kusakai G, Kishimoto A, Shimojo Y, Miyamoto S, Ogura T, Ochiai A, Esumi H.
Oncogene 2004 Sep; 23(42):7067.
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Early N-terminal changes and caspase-6 cleavage of tau in Alzheimer's disease.
Horowitz PM, Patterson KR, Guillozet-Bongaarts AL, Reynolds MR, Carroll CA, Weintraub ST, Bennett DA, Cryns VL, Berry RW, Binder LI.
Journal of Neuroscience 2004 Sep; 24(36):7895.
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Breaking the connection: caspase 6 disconnects intermediate filament-binding domain of periplakin from its actin-binding N-terminal region.
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