Products

Western Blot (Cell lysate)

Western blot analysis of CARD8 in tumor cell lines. Using CARD8 polyclonal antibody (Cat # PAB0281) at 1 : 2000. Cell lysates were normalized for total protein content.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Formalin-fixed, paraffin-embedded tissue human colon microarray stained for CARD8 expression at 1 : 2000. Using CARD8 polyclonal antibody (Cat # PAB0281) . A-A2, sucessively higher magnifications of the microarray. A, low magnification overview. A1, differential CARD8 staining between tissue cores is observed. A2, Areas of intense CARD8 staining in a colon carcinoma tissue core. Hematoxylin eosin counterstain.

Immunohistochemistry

Formalin-fixed, paraffin-embedded tumor/normal adjacent tissue cores from a human colon tissue microarray stained for CARD8 expression at 1:2000 using CARD8 polyclonal antibody (Cat # PAB0281).
A and B are tumor tissue cores.
A1 and B1 are the matched normal adjacent cores from A and B, respectively.
Hematoxylin-eosin counterstain.

Immunoprecipitation

Entrez GeneID

22900

Gene Name

CARD8

Gene Alias

CARDINAL, DACAR, DKFZp779L0366, Dakar, FLJ18119, FLJ18121, KIAA0955, MGC57162, NDPP, NDPP1, TUCAN

Gene Description

caspase recruitment domain family, member 8

Omim ID

609051

Gene Ontology

Hyperlink

Gene Summary

The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined. [provided by RefSeq

Other Designations

CARD inhibitor of NF-kappaB-activating ligands|CARD8 isoform T47|CARD8 isoform T51|CARD8 isoform T60|apoptotic protein NDPP1|tumor up-regulated CARD-containing antagonist of caspase nine