TRAF6 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of TRAF6 in normal human tissues. Using TRAF6 polyclonal antibody (Cat # PAB0279) at 1 : 2000. TRAF6 is observed at ~66 KDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF6 degradation fragments.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed, paraffin- embedded tissue sections stained for TRAF6 using TRAF6 polyclonal antibody (Cat # PAB0279) at 1 : 2000. Hematoxylin-eosin counterstain. A, mouse spleen. B, mouse thymus.
Immunohistochemistry
Formalin-fixed, paraffin- embedded tissue sections stained for TRAF6 using TRAF6 polyclonal antibody (Cat # PAB0279) at 1:2000.
Hematoxylin-eosin counterstain.
A, mouse lymph node.
B, mouse bone marrow.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of TRAF6.
Immunogen
A synthetic peptide corresponding to amino acids 451-469 of human TRAF6.
Sequence
DAKPELLAFQRPTIPRNPK
Host
Rabbit
Reactivity
Bovine, Human, Mouse, Rat
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of TRAF6 in normal human tissues. Using TRAF6 polyclonal antibody (Cat # PAB0279) at 1 : 2000. TRAF6 is observed at ~66 KDa. Additional bands of lower molecular weight were seen in some cases, and may represent TRAF6 degradation fragments.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed, paraffin- embedded tissue sections stained for TRAF6 using TRAF6 polyclonal antibody (Cat # PAB0279) at 1 : 2000. Hematoxylin-eosin counterstain. A, mouse spleen. B, mouse thymus.Immunohistochemistry
Formalin-fixed, paraffin- embedded tissue sections stained for TRAF6 using TRAF6 polyclonal antibody (Cat # PAB0279) at 1:2000.
Hematoxylin-eosin counterstain.
A, mouse lymph node.
B, mouse bone marrow.Immunoprecipitation
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Gene Info — TRAF6
Entrez GeneID
7189Gene Name
TRAF6
Gene Alias
MGC:3310, RNF85
Gene Description
TNF receptor-associated factor 6
Omim ID
602355Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported. [provided by RefSeq
Other Designations
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Interactome
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Pathway
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Disease
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Publication Reference
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Altered expression of microRNA miR-146a correlates with the development of chronic renal inflammation.
Ichii O, Otsuka S, Sasaki N, Namiki Y, Hashimoto Y, Kon Y.
Kidney International 2012 Feb; 81(3):280.
Application：WB-Tr, Human, THP-1 cells.
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TRAF6 promotes atypical ubiquitination of mutant DJ-1 and alpha-synuclein and is localized to Lewy bodies in sporadic Parkinson's disease brains.
Zucchelli S, Codrich M, Marcuzzi F, Pinto M, Vilotti S, Biagioli M, Ferrer I, Gustincich S.
Human Molecular Genetics 2010 Oct; 19(19):3759.
Application：IF, IHC, Human, Brains, Substantia nigra.
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TNFR-associated factor family protein expression in normal tissues and lymphoid malignancies.
Zapata JM, Krajewska M, Krajewski S, Kitada S, Welsh K, Monks A, McCloskey N, Gordon J, Kipps TJ, Gascoyne RD, Shabaik A, Reed JC.
Journal of Immunology 2000 Nov; 165(9):5084.
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Altered expression of microRNA miR-146a correlates with the development of chronic renal inflammation.
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