Western blot analysis of PTPN13. Using PTPN13 polyclonal antibody (Cat # PAB0256) at 1 : 2000. In ovarian carcinoma cell lines, PTPN13 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.
Immunohistochemisty of PTPN13 in formalin-fixed, paraffin embedded ovarian carcinoma cores from a tissue microarray. Using PTPN13 polyclonal antibody (Cat # PAB0256) at 1 : 2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-eosin counterstain.
Immunohistochemistry (Frozen sections)
Immunocytochemical analysis of PTPN13 in cell lines. Using PTPN13 polyclonal antibody (Cat # PAB0256) at 1 : 2000. In ovarian carcinoma cell lines PTPN13 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively. The staining data correlates with the western blot data (figure to the left) .
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large intracellular protein. It has a catalytic PTP domain at its C-terminus and two major structural domains: a region with five PDZ domains and a FERM domain that binds to plasma membrane and cytoskeletal elements. This PTP was found to interact with, and dephosphorylate, Fas receptor and IkappaBalpha through the PDZ domains. This suggests it has a role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathways. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq
APO-1/CD95 (Fas)-associated phosphatase,Fas-associated phosphatase-1,protein tyrosine phosphatase 1E,protein tyrosine phosphatase, non-receptor type 13,protein tyrosine phosphatase, nonreceptor type 13,protein-tyrosine phosphatase 1, Fas-associated,protei