PTPN13 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant PTPN13.
Immunogen
Recombinant protein corresponding to amino acids 1279- 1883 of human PTPN13.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of PTPN13. Using PTPN13 polyclonal antibody (Cat # PAB0256) at 1 : 2000. In ovarian carcinoma cell lines, PTPN13 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human Jurkat T and 293 kidney cell lines were used as negative and positive controls, respectively.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemisty of PTPN13 in formalin-fixed, paraffin embedded ovarian carcinoma cores from a tissue microarray. Using PTPN13 polyclonal antibody (Cat # PAB0256) at 1 : 2000. A-D, samples are from four different patients. A1-D1 are high magnification images from A-D, respectively. Hematoxylin-eosin counterstain.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemical analysis of PTPN13 in cell lines. Using PTPN13 polyclonal antibody (Cat # PAB0256) at 1 : 2000. In ovarian carcinoma cell lines PTPN13 expression was detected in SK-OV-3 and OVCAR-3, but not in BG-1 or HEY. Human 293 kidney and Jurkat T cell lines were used as positive and negative controls, respectively. The staining data correlates with the western blot data (figure to the left) .Immunofluorescence
Immunoprecipitation
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Gene Info — PTPN13
Entrez GeneID
5783Gene Name
PTPN13
Gene Alias
DKFZp686J1497, FAP-1, PNP1, PTP-BAS, PTP-BL, PTP1E, PTPL1, PTPLE
Gene Description
protein tyrosine phosphatase, non-receptor type 13 (APO-1/CD95 (Fas)-associated phosphatase)
Omim ID
600267Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP is a large intracellular protein. It has a catalytic PTP domain at its C-terminus and two major structural domains: a region with five PDZ domains and a FERM domain that binds to plasma membrane and cytoskeletal elements. This PTP was found to interact with, and dephosphorylate, Fas receptor and IkappaBalpha through the PDZ domains. This suggests it has a role in Fas mediated programmed cell death. This PTP was also shown to interact with GTPase-activating protein, and thus may function as a regulator of Rho signaling pathways. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported. [provided by RefSeq
Other Designations
APO-1/CD95 (Fas)-associated phosphatase|Fas-associated phosphatase-1|protein tyrosine phosphatase 1E|protein tyrosine phosphatase, non-receptor type 13|protein tyrosine phosphatase, nonreceptor type 13|protein-tyrosine phosphatase 1, Fas-associated|protei
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Interactome
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Disease
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Publication Reference
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Necl-4 enhances the PLCγ-c-Raf-MEK-ERK pathway without affecting internalization of VEGFR2.
Yamana S, Tokiyama A, Fujita H, Terao Y, Horibe S, Sasaki N, Satomi-Kobayashi S, Hirata KI, Rikitake Y.
Biochemical and Biophysical Research Communications 2017 Jun; 490(2):169.
Application:WB-Tr, Human, HUVEC.
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Expression and potential role of Fas-associated phosphatase-1 in ovarian cancer.
Meinhold-Heerlein I, Stenner-Liewen F, Liewen H, Kitada S, Krajewska M, Krajewski S, Zapata JM, Monks A, Scudiero DA, Bauknecht T, Reed JC.
The American Journal of Pathology 2001 Apr; 158(4):1335.
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Necl-4 enhances the PLCγ-c-Raf-MEK-ERK pathway without affecting internalization of VEGFR2.
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