Bid polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot analysis of Bid in normal mouse tissues using Bid polyclonal antibody (Cat # PAB0238) at 1 : 2000. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 KDa Bid and the ~15 KDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using Bid polyclonal antibody (Cat # PAB0238) at 1 : 2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.
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Specification
Product Description
Rabbit polyclonal antibody raised against full length recombinant Bid.
Immunogen
Recombinant protein corresponding to full length mouse Bid.
Host
Rabbit
Reactivity
Dog, Gerbils, Human, Mouse, Rat
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In serum (0.05% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot analysis of Bid in normal mouse tissues using Bid polyclonal antibody (Cat # PAB0238) at 1 : 2000. BID = recombinant Bid. Arrowheads indicate the positions of the full-length (uncleaved) ~22 KDa Bid and the ~15 KDa truncated form of Bid (tBid) typical of the caspase-cleavage. Additional bands representing partial Bid degradation products are indicated by asterisks (*).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue array stained for Bid expression using Bid polyclonal antibody (Cat # PAB0238) at 1 : 2000. Hematoxylin-eosin counterstain. Variable Bid expression is seen between patient samples.Immunohistochemistry (Frozen sections)
Immunoprecipitation
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Gene Info — Bid
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Publication Reference
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A novel canine B-cell leukaemia cell line. Establishment, characterisation and sensitivity to chemotherapeutics.
Pawlak A, Ziolo E, Kutkowska J, Blazejczyk A, Wietrzyk J, Krupa A, Hildebrand W, Dziegiel P, Dzimira S, Obminska-Mrukowicz B, Strzadala L, Rapak A.
Veterinary and Comparative Oncology 2016 Aug; [Epub].
Application：WB, Dog, CBL70, CLBl-1, CL-1, CLB70 cells and PBMCs.
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Increased placental apoptosis in maternal food restricted gestations: role of the Fas pathway.
Belkacemi L, Chen CH, Ross MG, Desai M.
Placenta 2009 Sep; 30(9):739.
Application：WB-Ti, Rat, Rat placenta.
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Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation.
Krajewska M, Rosenthal RE, Mikolajczyk J, Stennicke HR, Wiesenthal T, Mai J, Naito M, Salvesen GS, Reed JC, Fiskum G, Krajewski S.
Experimental Neurology 2004 Oct; 189(2):261.
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A novel canine B-cell leukaemia cell line. Establishment, characterisation and sensitivity to chemotherapeutics.
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