CCR1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CCR1.
Immunogen
A synthetic peptide corresponding to amino acids 339-350 of human CCR1.
Sequence
VSSTSPSTGEHE
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Recommend Usage
Flow Cytometry (5-10 ul)
Western Blot (1-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% gelatin, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 10 ug of total cell lysate from A-375 cells with CCR1 polyclonal antibody (Cat # PAB0176) at 2 ug/mL dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-Fixed Paraffin-Embedded Sections) staining of human spleen with CCR1 polyclonal antibody (Cat # PAB0176).Flow Cytometry
Left image : Surface staining of mouse primary epidermal cells (B-6 mice, 30 wks) with CL 11-PE, CD11c PE-Cy5, and CCR1 polyclonal antibody (Cat # PAB0176) with CY5 goat anti rabbit secondary. Right image : CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 uL (blue histogram) and 10 uL (Green histogram). Data kindly provided by Corey Parlet, University of Iowa. -
Gene Info — CCR1
Entrez GeneID
1230Protein Accession#
BAA01723.1Gene Name
CCR1
Gene Alias
CD191, CKR-1, CKR1, CMKBR1, HM145, MIP1aR, SCYAR1
Gene Description
chemokine (C-C motif) receptor 1
Omim ID
601159Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. The ligands of this receptor include macrophage inflammatory protein 1 alpha (MIP-1 alpha), regulated on activation normal T expressed and secreted protein (RANTES), monocyte chemoattractant protein 3 (MCP-3), and myeloid progenitor inhibitory factor-1 (MPIF-1). Chemokines and their receptors mediated signal transduction are critical for the recruitment of effector immune cells to the site of inflammation. Knockout studies of the mouse homolog suggested the roles of this gene in host protection from inflammatory response, and susceptibility to virus and parasite. This gene and other chemokine receptor genes, including CCR2, CCRL2, CCR3, CCR5 and CCXCR1, are found to form a gene cluster on chromosome 3p. [provided by RefSeq
Other Designations
RANTES receptor
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Interactome
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Pathway
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Disease
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Publication Reference
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Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors.
Kurashima Y, Amiya T, Nochi T, Fujisawa K, Haraguchi T, Iba H, Tsutsui H, Sato S, Nakajima S, Iijima H, Kubo M, Kunisawa J, Kiyono H.
Nature Communications 2012 Sep; 3:1034.
Application:Flow Cyt, Mouse, ECs and lamina propria mononuclear cells.
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CCR1/CCL5 (RANTES) receptor-ligand interactions modulate allogeneic T-cell responses and graft-versus-host disease following stem-cell transplantation.
Choi SW, Hildebrandt GC, Olkiewicz KM, Hanauer DA, Chaudhary MN, Silva IA, Rogers CE, Deurloo DT, Fisher JM, Liu C, Adams D, Chensue SW, Cooke KR.
Blood 2007 Nov; 110(9):3447.
Application:Flow Cyt, Mouse , Mouse splenocytes.
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Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors.
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