IPO9 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant IPO9.
Immunogen
Recombinant protein corresponding to amino acids 950-1000 of human IPO9.
Host
Rabbit
Reactivity
Bovine, Chimpanzee, Human, Monkey, Mouse, Rat
Form
Liquid
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS (0.05% BSA, 0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Importin-9 in HeLa lysate in the 1) absence and 2) presence of immunizing peptide. Using IPO9 polyclonal antibody (Cat # PAB0154) at 0.5 ug/mL .Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
IHC analysis of human brain, cortex. Using IPO9 polyclonal antibody (Cat # PAB0154) at 10 ug/mL . -
Gene Info — IPO9
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Interactome
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Publication Reference
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Regulation of nuclear actin levels and MRTF/SRF target gene expression during PC6.3 cell differentiation.
Salla Kyheröinen, Alise Hyrskyluoto, Maria Sokolova, Maria K Vartiainen.
Experimental Cell Research 2022 Nov; 420(2):113356.
Application:WB-Ce, Rat, PC6.3 cells.
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Nuclear actin modulates cell motility via transcriptional regulation of adhesive and cytoskeletal genes.
Sharili AS, Kenny FN, Vartiainen MK, Connelly JT.
Scientific Reports 2016 Sep; 6:33893.
Application:WB, IF, Human, HaCaT cells.
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Active maintenance of nuclear actin by importin 9 supports transcription.
Dopie J, Skarp KP, Rajakylä EK, Tanhuanpää K, Vartiainen MK.
PNAS 2012 Feb; 109(9):E544.
Application:WB-Tr, Drosophila, S2R+ cells.
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Regulation of nuclear actin levels and MRTF/SRF target gene expression during PC6.3 cell differentiation.
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